American Association for Cancer Research
00085472can132441-sup-fig3.pdf (229.81 kB)

Supplementary Figure 3 from Phosphatidylinositol 4-Phosphate in the Golgi Apparatus Regulates Cell–Cell Adhesion and Invasive Cell Migration in Human Breast Cancer

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journal contribution
posted on 2023-03-30, 22:04 authored by Emi Tokuda, Toshiki Itoh, Junya Hasegawa, Takeshi Ijuin, Yukiko Takeuchi, Yasuhiro Irino, Miki Fukumoto, Tadaomi Takenawa

PDF file - 229K, Supplementary Figure 3. Knockdown of SAC1 and PI4KIIIbeta in MCF7 cells.



Downregulation of cell–cell adhesion and upregulation of cell migration play critical roles in the conversion of benign tumors to aggressive invasive cancers. In this study, we show that changes in cell–cell adhesion and cancer cell migration/invasion capacity depend on the level of phosphatidylinositol 4-phosphate [PI(4)P] in the Golgi apparatus in breast cancer cells. Attenuating SAC1, a PI(4)P phosphatase localized in the Golgi apparatus, resulted in decreased cell–cell adhesion and increased cell migration in weakly invasive cells. In contrast, silencing phosphatidylinositol 4-kinase IIIβ, which generates PI(4)P in the Golgi apparatus, increased cell–cell adhesion and decreased invasion in highly invasive cells. Furthermore, a PI(4)P effector, Golgi phosphoprotein 3, was found to be involved in the generation of these phenotypes in a manner that depends on its PI(4)P-binding ability. Our results provide a new model for breast cancer cell progression in which progression is controlled by PI(4)P levels in the Golgi apparatus. Cancer Res; 74(11); 3054–66. ©2014 AACR.