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00085472can076560-sup-sfig_2.pdf (252.68 kB)

Supplementary Figure 2 from Novel Role of Thromboxane Receptors β Isoform in Bladder Cancer Pathogenesis

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posted on 2023-03-30, 18:10 authored by Omar Moussa, Anthony W. Ashton, Mostafa Fraig, Elizabeth Garrett-Mayer, Mohamed A. Ghoneim, Perry V. Halushka, Dennis K. Watson
Supplementary Figure 2 from Novel Role of Thromboxane Receptors β Isoform in Bladder Cancer Pathogenesis

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ARTICLE ABSTRACT

These studies were undertaken to determine the potential role of thromboxane receptors (TP) in bladder cancer. The data reported herein show that expression of the TP-β receptor protein is increased in tissue obtained from patients with bladder cancer and associated with a significantly poorer prognosis (P < 0.005). Bladder cancer cell lines express the TP-β isoform, unlike immortalized nontransformed urothelial cells (SV-HUC) that express only the TP-α isoform. TP-β receptor expression, but not TP-α, promoted cell proliferation, migration, and invasion in vitro, and also resulted in malignant transformation of SV-HUC cells in vivo. Agonist-mediated phosphorylation of extracellular signal-regulated kinase and FAK was dependent on the expression of TP-β. Furthermore, TP-β mediated multiple biological effects by signaling through either G-protein α subunit 12 or β-arrestin 2. Treatment of mice with the TP receptor antagonist GR32191, alone or in combination with cisplatin, significantly delayed tumor onset and prolonged survival of mice transplanted with TCC-SUP bladder cancer cells compared with vehicle or cisplatin alone. These results support the model that the TP-β receptor isoform plays a unique role in bladder cancer progression and its expression may have predictive value and provide a novel therapeutic target. [Cancer Res 2008;68(11):4097–104]

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