American Association for Cancer Research
21598290cd201274-sup-250305_2_supp_6853956_qn4tzm.pdf (6.32 MB)

Supplementary Figure 2 from Nongenetic Evolution Drives Lung Adenocarcinoma Spatial Heterogeneity and Progression

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journal contribution
posted on 2023-04-03, 23:44 authored by Daniele Tavernari, Elena Battistello, Elie Dheilly, Aaron S. Petruzzella, Marco Mina, Jessica Sordet-Dessimoz, Solange Peters, Thorsten Krueger, David Gfeller, Nicolo Riggi, Elisa Oricchio, Igor Letovanec, Giovanni Ciriello

Supplementary Figure 2


Gabriella Giorgi-Cavaglieri foundation

University of Lausanne Interdisciplinary Research Grant

Swiss National Science Foundation



Cancer evolution determines molecular and morphologic intratumor heterogeneity and challenges the design of effective treatments. In lung adenocarcinoma, disease progression and prognosis are associated with the appearance of morphologically diverse tumor regions, termed histologic patterns. However, the link between molecular and histologic features remains elusive. Here, we generated multiomics and spatially resolved molecular profiles of histologic patterns from primary lung adenocarcinoma, which we integrated with molecular data from >2,000 patients. The transition from indolent to aggressive patterns was not driven by genetic alterations but by epigenetic and transcriptional reprogramming reshaping cancer cell identity. A signature quantifying this transition was an independent predictor of patient prognosis in multiple human cohorts. Within individual tumors, highly multiplexed protein spatial profiling revealed coexistence of immune desert, inflamed, and excluded regions, which matched histologic pattern composition. Our results provide a detailed molecular map of lung adenocarcinoma intratumor spatial heterogeneity, tracing nongenetic routes of cancer evolution. Lung adenocarcinomas are classified based on histologic pattern prevalence. However, individual tumors exhibit multiple patterns with unknown molecular features. We characterized nongenetic mechanisms underlying intratumor patterns and molecular markers predicting patient prognosis. Intratumor patterns determined diverse immune microenvironments, warranting their study in the context of current immunotherapies.This article is highlighted in the In This Issue feature, p. 1307

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