posted on 2023-04-03, 14:00authored byQie Guo, Peixiang Lan, Xin Yu, Qiuju Han, Jian Zhang, Zhigang Tian, Cai Zhang
PDF - 159K, Figure S2. shRNA and bi-functional vector suppresses the expression of anti-apoptotic genes in hepatoma cells. A, The expressions of apoptosis-related genes Pim-3, Bcl-XL, Bcl-2, and Bim in Hepa1-6 cells were detected by RT-PCR and Western blotting. B, The expressions of Pim-3, Bad, and phospho-Bad were detected by Western blotting. The relative expression of pim-3 was normalized to that of beta-actin, and that of phospho-Bad to that of total Bad. C, Concentration of caspase-8 in cell lysates of Hepa1-6 after transfection for 24 h with the indicated vectors, as measured by ELISA. Data are representative or means (plus or minus) SD of three independent experiments. **, P < 0.01, compared with the pSIREN transcription group.