American Association for Cancer Research
Browse
00085472can076353-sup-sfig_2.pdf (189.22 kB)

Supplementary Figure 2 from Identification of Tumor-Initiating Cells in a p53-Null Mouse Model of Breast Cancer

Download (189.22 kB)
journal contribution
posted on 2023-03-30, 17:42 authored by Mei Zhang, Fariba Behbod, Rachel L. Atkinson, Melissa D. Landis, Frances Kittrell, David Edwards, Daniel Medina, Anna Tsimelzon, Susan Hilsenbeck, Jeffrey E. Green, Aleksandra M. Michalowska, Jeffrey M. Rosen
Supplementary Figure 2 from Identification of Tumor-Initiating Cells in a p53-Null Mouse Model of Breast Cancer

History

ARTICLE ABSTRACT

Using a syngeneic p53-null mouse mammary gland tumor model that closely mimics human breast cancer, we have identified, by limiting dilution transplantation and in vitro mammosphere assay, a Lin−CD29HCD24H subpopulation of tumor-initiating cells. Upon subsequent transplantation, this subpopulation generated heterogeneous tumors that displayed properties similar to the primary tumor. Analysis of biomarkers suggests the Lin−CD29HCD24H subpopulation may have arisen from a bipotent mammary progenitor. Differentially expressed genes in the Lin−CD29HCD24H mouse mammary gland tumor-initiating cell population include those involved in DNA damage response and repair, as well as genes involved in epigenetic regulation previously shown to be critical for stem cell self-renewal. These studies provide in vitro and in vivo data that support the cancer stem cell (CSC) hypothesis. Furthermore, this p53-null mouse mammary tumor model may allow us to identify new CSC markers and to test the functional importance of these markers. [Cancer Res 2008;68(12):4674–82]

Usage metrics

    Cancer Research

    Categories

    Keywords

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC