Supplementary Figure 2 from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis

PDF - 202K, (A) Representative images of wound healing assay of MDA-MB-231 cells. Right after the wound was made on the MDA-MB-231 cell monolayer, the cells were treated with TGF-beta1 (2 ng/ml) with or without ALK5 inhibitors for 53 h. Total magnification was x 100. Scale bar indicates 100 microm. Veh, SB, and EW indicate vehicle, SB-505124, and EW-7197, respectively. (B, C) Effects of EW-7197 on cell cytotoxicity (described in the Supplementary Methods). 4T1 (B) and MCF10A (C) cells were treated with indicated concentrations of EW-7197 in 0.2% HI-FBS medium for 72 h. The cell viability was determined by SRB (Sulforhodamine B) assay (described in the Supplementary Methods). Data represent the mean (plus or minus) SD (n=3).