posted on 2023-04-03, 16:06authored byGregor M. Balaburski, Julia I.-Ju Leu, Neil Beeharry, Seth Hayik, Mark D. Andrake, Gao Zhang, Meenhard Herlyn, Jessie Villanueva, Roland L. Dunbrack, Tim Yen, Donna L. George, Maureen E. Murphy
PDF file - 280K, A) Chemical structures of PES and synthesized derivatives. P=pyrrolidine; CM= carboxymethyl; Cl=chloride. B) MTT cell viability analyses of H1299 and PANC1 cells after 48 hour treatment with 10 uM PES, PES-P, PES-PCM and PES-PCl. The values shown are the averaged results from three independent experiments, and error bars mark standard deviations. C) Immunoblot analysis of H1299 cells following treatment with 10 uM of the compounds indicated for the timepoints indicated, using antisera to the autophagy (p62SQSTM1 and LC3) and apoptosis (cleaved lamin A) markers indicated, as well as HSP70 and actin (control). The arrows mark monomeric and oligomeric forms of p62SQSTM1, as well as cleaved and lipidated forms of LC3 I and II.