E-Cadherin Regulates Human Nanos 1 , which Interacts with p 120 ctn and Induces Tumor Cell Migration and Invasion

Down-regulation of the epithelial cell-cell adhesion molecule E-cadherin is frequently associated with tumor formation and progression. Besides its role in physical cell-cell adhesion, E-cadherin is also thought to be involved in intracellular signaling in normal epithelial cells. In these cells, the Armadillo catenin p120ctn binds to the cytoplasmic domain of E-cadherin and stabilizes the adhesion complexes. On loss of E-cadherin, cytoplasmic p120ctn might accumulate and contribute to tumor malignancy. We used suppression subtractive hybridization to search for genes regulated by E-cadherin expression. We isolated human Nanos1 as a transcript of which levels decrease on E-cadherin reexpression in a human breast cancer cell line. The hNanos1 protein bears a COOH-terminal (CCHC)2 zinc finger domain and belongs to an evolutionarily conserved protein family sharing functions in germ cell development in both vertebrates and invertebrates. We found an inverse correlation between E-cadherin and hNanos1 expression in various cell lines and under diverse conditions. Conditional expression of hNanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion. It induced cytoplasmic translocation of p120ctn, as well as strong migratory and invasive properties. We also found that the NH2-terminal domain of hNanos1, which is conserved only among mammals, interacts with p120ctn. hNanos1 counteracted the stimulatory effect of p120ctn on cell protrusion formation. Together, these findings describe a new function for hNanos1 as a downstream effector of E-cadherin loss contributing to tumor progression. Targeting hNanos1 might be a promising strategy in the treatment of E-cadherin–negative tumors in particular. (Cancer Res 2006; 66(20): 10007-15)


Introduction
E-Cadherin is a major epithelial cell-cell adhesion molecule that functions as a tumor suppressor (1).Moreover, E-cadherin deficiency plays a causative role in tumor cell invasion and metastasis (2)(3)(4).The extracellular domains of the transmembrane E-cadherin molecules form homophilic adhesion dimers stabilized by direct binding to cytoplasmic Armadillo catenins (5).h-Catenin interacts tightly via its Armadillo repeats with the catenin binding domain of the COOH-terminal tail of E-cadherin and via its NH 2terminal domain with a-catenin.This is essential for the formation of actin cytoskeleton-coupled cell junctions, although the detailed mechanism is a matter of debate (6).In addition, p120 catenin (p120ctn) associates via its Armadillo repeat region with the juxtamembrane domain of the E-cadherin cytoplasmic tail.The interactions of E-cadherin with p120ctn and h-catenin are necessary for dynamically regulating adequate cell-cell adhesion by modulating events such as cadherin clustering and the strength of the connection with the actin cytoskeleton (reviewed in ref. 5).One major function of p120ctn is to stabilize cadherin complexes at the cell membrane by controlling the entry of cadherins into the degradative endocytic pathway (reviewed in refs 7, 8).
The molecular basis of the suppressive signals that are conferred by functional expression of E-cadherin on cancer cells has not been fully elucidated.Loss of E-cadherin might induce signaling by catenins released from the cell membrane.Indeed, h-catenin is also a major component of the canonical Wnt signaling pathway (reviewed in refs.5,9).When the Wnt signaling pathway is turned on (e.g., by certain gene mutations in cancer), cytoplasmic hcatenin becomes protected from proteasomal degradation, translocates to the nucleus, binds LEF/Tcf, and acts as an oncogenic transcription cofactor.However, in the absence of such mutations or of a Wnt signal, the h-catenin that is released from the membrane on cadherin loss is rapidly degraded.Cadherins are both necessary and sufficient for localization of p120ctn at the cell membrane (10).Cadherin deficiency does not significantly alter p120ctn levels but leaves it stranded in the cytoplasm and/or the nucleus (10)(11)(12).In the cytoplasm, p120ctn interacts with Rho GTPases (reviewed in refs.7,8,13).It directly binds, via its Armadillo repeats, to RhoA and inhibits its activity by acting as a guanine nucleotide dissociation inhibitor.p120ctn also indirectly activates Rac1 and Cdc42 through guanine nucleotide exchange factors such as Vav-2.The combined effects of cytoplasmic p120ctn promote cell migration and, consequently, invasion and metastasis.In the nucleus, p120ctn can interact with the transcription factor Kaiso, and it is likely that this also contributes to the malignant phenotype (14,15).
Here, we describe the identification of human Nanos1 as a transcript that is down-regulated by E-cadherin.The human Nanos 1 gene encodes a (CCHC) 2 zinc finger protein that is highly homologous to the Nanos orthologues in Drosophila and mouse, which are known to have an evolutionarily conserved function in embryonic patterning and germ line development (16)(17)(18)(19)(20)(21)(22).Our finding that E-cadherin regulates the expression of hNanos1 in carcinoma cells prompted us to seek a functional link between Ecadherin and hNanos1 in epithelial cells.We generated cell systems with inducible expression of hNanos1 and found that hNanos1 impairs E-cadherin function in cell-cell adhesion and promotes cell motility and invasion.Interestingly, we found that hNanos1 interacts with the Armadillo protein p120ctn and counteracts the effect of its overexpression on cell protrusion formation.

Materials and Methods
Suppression subtractive hybridization and cloning of hNanos1.Polyadenylated mRNA, obtained from subconfluent human E-cadherinnegative MDA-MB-231 breast cancer cells and a derivative transfected cell line reexpressing E-cadherin, was isolated with the Fast Track 2.0 Kit (Invitrogen, Merelbeke, Belgium), in accordance with the recommendations of the manufacturer.cDNA subtraction was done using the PCR-select cDNA subtraction kit (BD Biosciences Clontech, Erembodegem, Belgium).The subtracted cDNA libraries, generated by subcloning the PCR products in the pGEM-T vector (Promega, Madison, WI), were screened for differentially expressed cDNAs using plus/minus colony hybridization.Finally, the differential expression of isolated clones was confirmed by Northern blotting.
The 538-bp hNanos1 cDNA fragment isolated in the suppression subtractive hybridization (SSH) screen was used to probe a fetal kidney phage cDNA library (Clontech).The full-length sequence of the hNanos1 cDNA was obtained by sequencing the isolated specific cDNA clones and several EST clones that were identified by BLAST analysis.To identify the 5 ¶ and 3 ¶ ends of the hNanos1-specific transcripts, rapid amplification of cDNA ends (RACE) was done on RNA isolated from SW480 human colon cancer cells.Additional genomic sequence data were obtained by isolation and characterization of a hNanos1-specific genomic PAC clone.A 16-kb HindIII fragment containing the single-exon hNanos1 gene was subcloned into the pGEM-11Zf(+) vector (Promega) to produce pGEM11-hNanos1PAC74-HindIII.
Northern blot analysis.Total RNA was isolated from various human cell lines with the RNeasy kit (Qiagen, Chatsworth, CA) according to the instructions of the manufacturer.Total RNA (30 Ag) was glyoxylated, size fractionated in a 1% agarose gel, and transferred to a Hybond-N + membrane (Amersham Pharmacia Biotech, Rainham, United Kingdom).Hybridization was done following standard procedures.Probes were radioactively labeled by random priming (RadPrime labeling kit, Gibco BRL Life Technologies, Paisley, United Kingdom).The hNanos1-specific probe used was the 538-bp insert of the pGEM-T clone obtained from the SSH analysis.The other probe was an 800-bp fragment (exons 14-16) of the human E-cadherin cDNA.To control for the amounts of RNA loaded, a probe specific for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used in an additional hybridization on the same blot.Radioactive bands were quantified with a PhosphorImager (Molecular Imager FX, Bio-Rad, Richmond, CA).
Cell aggregation assay.Single-cell suspensions were prepared according to an E-cadherin saving procedure (26).Cells were incubated for 30 minutes with continuous shaking in an isotonic buffer containing 1.25 mmol/L Ca 2+ .Rat mAb DECMA-1 (Sigma) was used to functionally block E-cadherin, starting 30 minutes before aggregation at 4jC and continuing throughout aggregation at 37jC.Particle diameters were measured with a Coulter particle size counter LS200 (Coulter Electronics

Cancer Research
Cancer Res 2006; 66: (20).October 15, 2006 Ltd., Miami, FL) at the start of the incubation at 37jC and after 30 minutes of incubation.The results were plotted against the volume distribution (expressed as percent of the total cell volume).Statistical differences were determined by applying the Kolmogorov-Smirnov test to cumulative fast aggregation curves.
Collagen type I invasion assay.Cells were seeded on top of a jellified collagen type I solution (0.09%; Upstate Biotechology, Lake Placid, NY).Invasion was scored on living cultures using a microscope with a computercontrolled stage as described (27).
Cell dispersion assay.The spatial distribution of cells was characterized and quantified with an algorithmic program of cellular sociology based on three geometric models: Voronoi's partition, Delaunay's graph, and minimum spanning tree (28).Details are described in Supplementary data.
In vitro cell migration assay.For each cell type, 10 5 cells were seeded in culture medium in a 22-mm dish with a 6-mm restriction ring placed inside.Twenty-four hours after plating, the ring was removed, the cells were washed twice with PBS and then covered with culture medium with or without doxycycline.In this model, the cells migrate as an outgrowth from the confluent area initially delineated by the ring.Five hours after removing the ring, phase-contrast images of outgrowth areas were recorded at a Â40 magnification every 15 minutes during 1 hour.Cell migration was characterized and quantified with a previously described software program (29) that measures trajectories of peripheral cell nuclei and cell migration speeds.

Results
Cloning of hNanos1 mRNA as an E-cadherin-suppressed transcript.To identify genes the expression of which is modulated by E-cadherin expression, we conducted comparative transcriptome analysis of E-cadherin-negative and E-cadherin-positive cancer cell lines.Through SSH analysis of the closely related human breast cancer cell line MDA-MB-231 and its E-cadherinexpressing counterpart, MDA2BE5.36 (11,28), we isolated a 538-bp cDNA fragment the expression of which was lower in the E-cadherin-expressing cells.Completion of the 4,035-nucleotide (nt) full-length cDNA sequence (GenBank accession no.AF458985), which we originally called hEC-Rep1a for human E-cadherin repressed 1a, showed that the originally cloned cDNA fragment was part of the 3 ¶ untranslated region (UTR) of the human Nanos1 transcript (Fig. 1A), an orthologue of the Drosophila Nanos gene (16).According to the UCSC human genome browser (NCBI Build 35), 5 hNanos1 is a single-exon gene located on chromosome 10q26.11.Northern blot analysis confirmed the differential expression of hNanos1 in the MDA-MB-231 cell line relative to its E-cadherin expressing derivative MDA2BE5.36, and also revealed hybridization to two transcripts, one of 1,810 nt and one of 4,035 nt (Fig. 1B).These transcripts differ only in the size of their 3 ¶-UTR sequences due to the use of an alternative poly-adenylation signal (AAAAAA) at position 1,764 of the transcript (Fig. 1A), which was also confirmed by 3 ¶ RACE experiments (data not shown).
Alignment of the predicted hNanos1 ORF of 292-amino-acid residues with those of other Nanos-related proteins revealed a high degree of conservation of the COOH-terminal (CCHC) 2 zinc finger domain of 52 amino acids (Fig. 1C and Supplementary Fig. S1).In contrast, the sequences and lengths of their NH 2 -terminal parts were poorly conserved (data not shown), as had been described for several Nanos orthologues (30).
Restricted mRNA expression profile of hNanos1.Northern blot analysis of hNanos1 mRNA expression in a panel of Ecadherin-negative and E-cadherin-positive cell lines revealed that this transcript was not ubiquitously expressed.hNanos1 was expressed at high levels only in the E-cadherin-deficient cell lines SW480 (colon cancer), SK-BR-3 and MDA-MB-231 (breast cancer), SK-N-AS (neuroblastoma), and WERI-Rb-1 (retinoblastoma; Fig. 1D).Moderate expression levels were detected in the A431 (epidermoid), MDA2BE5.36 (breast cancer), and IMR32 (neuroblastoma) cell lines.hNanos1 transcripts were rare or absent in several other cell lines tested.In conclusion, high levels of hNanos1 mRNA were only seen in cell lines expressing low levels of E-cadherin mRNA.All hNanos1-positive cell lines analyzed expressed both the 1,810-nt and 4,035-nt transcripts.
hNanos1 and E-cadherin expression are inversely related.E-Cadherin expression can be modulated in the hNanos1-positive human colon cancer cell line SW480 by treatment with phorbol ester, by transfection with Smad4 cDNA, or by growing the cells to confluency (24,25).Treatment of SW480 cells with the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) led to a gradual decrease in hNanos1 mRNA levels during the first 6 hours of treatment, after which hNanos1 mRNA levels were reestablished (Fig. 2A).At the end point of TPA treatment (24 hours), hNanos1 mRNA levels were even higher than in untreated cells.E-Cadherin mRNA levels had an inverse profile, as they increased during the first 6 hours of treatment and then decreased to zero after 24 hours of TPA treatment.In Smad4transfected SW480 clones D1 and D14, E-cadherin mRNA expression was induced and hNanos1 mRNA expression was reduced (Fig. 2B) in comparison with E-cadherin-deficient mocktransfected SW480 clones K5 and K6.Finally, the inverse correlation between E-cadherin and hNanos1 mRNA expression was shown by culturing these cells at increasing cell densities, which resulted in induction of E-cadherin and reduction of hNanos1 with increasing cell density in both Smad4-and mocktransfected SW480 cells (Fig. 2C).
hNanos1 disturbs E-cadherin-dependent cell-cell adhesion.To determine the intracellular localization and function of hNanos1, we generated a human epithelial cancer cell system with inducible expression of Myc-tagged hNanos1.Addition of doxycycline induces tagged hNanos1 in DLD1-Teton-hNanos1 clones, but not in DLD1-Teton-mock clones, as shown by immunoblotting (55-kDa band in Fig. 3A).This induction was associated with an obvious morphotypic switch: cells lost the ability to form compact colonies and showed more and longer cytoplasmic protrusions (Fig. 3B), although total levels of the cell junctional proteins E-cadherin, p120ctn, and h-catenin were not decreased (Fig. 3C).
In a cell aggregation assay, noninduced DLD1-Teton-hNanos1 cells formed large aggregates within 30 minutes (Fig. 3D, Àdox N30).Aggregation required functional E-cadherin, as it was prevented by addition of an antibody (DECMA-1) that functionally blocks E-cadherin.When hNanos1 was induced by addition of doxycycline, the cells failed to form aggregates and their particle size distribution curve resembled that of cells treated with DECMA-1 (Fig. 3D, +dox N30).DLD1-Teton-mock cells showed aggregation after 30 minutes irrespective of doxycycline treatment (Fig. 3D).In summary, induction of hNanos1 in epithelial tumor cells interfered with their ability to form E-cadherin-dependent three-dimensional aggregates, although total levels of E-cadherin were not decreased.Immunofluorescence analysis revealed that induced Myc-tagged hNanos1 in DLD1-Teton-hNanos1 cells was expressed as cytoplasmic dots with perinuclear concentration (Fig. 3E, e).E-Cadherin, h-catenin, and p120ctn were highly enriched at cell-cell contacts in DLD1-Teton-mock cells irrespective of doxycycline treatment (data not shown), and a similar situation was seen in DLD1-Teton-hNanos1 cells not treated with doxycycline (Fig. 3E, a-d).However, expression of Myc-tagged hNanos1 induced less intense staining at the cell contacts for each of the three proteins investigated (Fig. 3E, f-h), and in the case of p120ctn, even prominent cytoplasmic staining was seen although nuclei remained always unstained (Fig. 3E, h).These results indicate that strong expression of hNanos1 interferes with the function of the E-cadherin junctional complex in human tumor cells by relocalizing the junctional proteins without altering their expression levels.
hNanos1 induces cell migration, cell dispersion, and invasion of epithelial cells.An in vitro cell migration model was used to determine whether induction of hNanos1 influences cell migration.We monitored the migration of DLD1-Teton-mock and DLD1-Teton-hNanos1 cells (with or without doxycycline) growing out from a confluent monolayer into adjacent free space.Phasecontrast images showed that 6 hours after the start of the experiment, induced DLD1-Teton-hNanos1 cells (+Dox) clearly had migrated a longer distance than untreated DLD1-Teton-hNanos1 cells and DLD1-Teton-mock cells (with or without doxycycline; Fig. 4A).Migration was characterized quantitatively in this assay.hNanos1 Induces Tumor Cell Migration and Invasion Migration trajectories of individual cells showed that the movements of DLD1-Teton-mock cells (untreated and treated) and untreated DLD1-Teton-hNanos1 cells were restricted and random, in contrast to the strikingly directional migration of the induced DLD1-Teton-hNanos1 cells (Fig. 4B).In addition, the migration speed was significantly increased by 81% on induction of hNanos1 expression in the DLD1-Teton-hNanos1 cells (29.23 F 7.56 Am/h) as compared with the untreated DLD1-Teton-hNanos1 cells (16.14 F 3.99 Am/h; Fig. 4C).No difference in cell migration speed between the untreated and treated DLD1-Teton-mock cells was observed.In conclusion, we show that hNanos1 induction in DLD1-Teton-hNanos1 cells results in a rapid and directional cell migration.
The changes in the migratory capacity of hNanos1-expressing cells were confirmed in a cell dispersion assay (ref.28; Supplementary Fig. S2).Induction of hNanos1 expression in DLD1-Teton-hNanos1 cells resulted in a more random spatial cell distribution of these cells compared with control cells, reflecting increased migratory and invasive properties of hNanos1-expressing cells.
To further evaluate whether hNanos1 expression correlates with invasion into a three-dimensional matrix, we did a collagen type-I invasion assay.Expression of hNanos1 strongly induced invasiveness in DLD1-Teton-hNanos1 cells, similar to that induced by the E-cadherin-blocking antibody DECMA-1 (Fig. 4D).
hNanos1 interacts with the Armadillo catenin p120ctn.In Drosophila, Nanos protein is recruited by Pumilio to the Nanos response elements in the 3 ¶-UTR of target mRNAs (31).The crystal structure of the Pumilio Puf domain, consisting of eight so-called PUM repeats forming a positively charged superhelix, resembles that of the Armadillo repeats (32).Based on the Nanos-Pumilio interaction and the topological similarities of the PUM and Armadillo repeats, we investigated the possibility that hNanos1 interacts with human Armadillo proteins.Myc-tagged Nanos indeed formed a complex with endogenous p120ctn in induced (+dox) DLD1-Teton-hNanos1 cells (Fig. 5A) but did not associate with the non-Armadillo repeat protein a-catenin (Fig. 5A) or with E-cadherin (data not shown), whereas a-catenin associated with E-cadherin as expected (not shown).These findings were consolidated and extended in Madin-Darby canine kidney-Tetoff-hNanos1 cells with inducible expression of hNanos1: reciprocal coimmunoprecipitations showed that hNanos1 interacts with both endogenous h-catenin and endogenous p120ctn (data not shown).To identify which part of hNanos1 mediated this interaction with p120ctn, we extended our analysis to HEK293T cells transiently cotransfected with constructs encoding full-length p120ctn (isoform 3A) and Myc-tagged hNanos1 ( full-length protein, NH 2terminal or COOH-terminal fragment).Interestingly, we found that the hNanos1-p120ctn interaction was mediated by the NH 2terminal part of hNanos1, which lacks the evolutionarily conserved zinc finger domain (Fig. 5B).
hNanos1 abolishes arborization caused by p120ctn overexpression.The observed interactions between hNanos1 and Armadillo proteins indicate that hNanos1 might affect the functions of these interaction partners.Because p120ctn is known to function in cell migration by modulating Rho GTPases in the cytoplasm (reviewed in ref. 13), we analyzed the effect of hNanos1 expression on this function.Overexpression of p120ctn in epithelial cells increases lamellipodia formation.This effect is particularly striking in fibroblasts, in which overexpressed p120ctn induces dramatic arborization (33).Therefore, we analyzed the effect of hNanos1 expression on p120ctn functionality in transfected NIH 3T3 fibroblasts.Exogenous p120ctn and hNanos1 both resided in the cytoplasm in these cells.Coexpression of either full-length or an NH 2 -terminal fragment of hNanos1 in NIH 3T3 cells expressing high levels of ectopic p120ctn diminished formation of dendritelike structures (Fig. 5C, b and c), whereas cotransfection of a backbone vector did not interfere with the phenotype induced by p120ctn (Fig. 5C, a).Notice in c and f that a cell that expresses EGFP-p120ctn but not the hNanos1 fragment still exhibits arborization, in contrast to a cell coexpressing both proteins.

Discussion
E-Cadherin expression is frequently down-regulated in many different types of tumors (34).Because E-cadherin is a key cell-cell adhesion protein in epithelial cells, loss of its expression or function diminishes cell-cell contacts and contributes to tumor progression and invasion.However, as E-cadherin inactivation may also occur early in certain precursor lesions, its loss is thought to induce intracellular signaling that promotes tumor development and progression.Because little is known about such signaling pathways, we screened for transcripts that are differentially expressed on E-cadherin reexpression in the breast cancer cell line MDA-MB-231.We isolated a partial cDNA that was downregulated in cells reexpressing E-cadherin.The completed fulllength cDNA sequence was highly homologous to the evolutionarily conserved gene Nanos.
All the Nanos and Nanos-related proteins that have been characterized in several organisms contain a conserved COOHterminal RNA-binding (CCHC) 2 zinc finger domain (Fig. 1C and Supplementary Fig. S1; ref. 20,21,30,35).In Drosophila, Nanos cooperates with Pumilio to repress translation by binding to Nanos response elements located in the 3 ¶-UTR of target transcripts (e.g., hunchback and cyclin B mRNAs; refs.36,37).Nanos regulates several processes in Drosophila, including differentiation of the anterior-posterior body axis (16), control of neuronal dendrite morphogenesis (38), establishment of germ cell identity (39), selfrenewal of germ line stem cells (40), migration and survival of primordial germ cells (17,18), oogenesis and spermatogenesis (19).Mice deficient in the individual Nanos genes are viable (20,21).Nanos1 knockout mice are fertile and exhibit no obvious phenotypic abnormalities.In contrast, Nanos2 is essential for germ cell development in male mice, and Nanos3 is important in both male and female sexual differentiation.A human Nanos protein was reported to interact with human Pumilio-2 and to be abundant in germ line stem cells (22).
In Drosophila, Nanos expression is regulated at the translational level (41).Whereas most Nanos mRNA is translationally repressed in the Drosophila embryo, translational activation occurs at the embryonic posterior pole.This switch from translational repression to activation is mediated by cis-acting signals in the 3 ¶-UTR of the Nanos mRNA (41,42).Whether Nanos expression can be translationally regulated also in mammals needs further investigation.Our identification of two hNanos1 transcripts that differ only in the lengths of their 3 ¶-UTR might be relevant to this.In E-cadherindeficient cancer cells, hNanos1 expression seems to be regulated at the transcriptional level.We cloned the minimal hNanos1 promoter in a luciferase reporter construct and found that reporter gene activity in MDA-MB-231 cells is higher than in the E-cadherin transfectant. 6This implies the presence of E-cadherin-sensitive regulatory sequences.
We found that expression of the hNanos1 transcript was reduced following E-cadherin reexpression in a human cancer cell line.Analysis of a series of cell lines showed an inverse correlation between E-cadherin and hNanos1 expression.In the human colorectal cancer cell line SW480, hNanos1 expression was inversely regulated by various conditions modulating E-cadherin expression.On TPA treatment, hNanos1 expression diminished during the first 6 hours, then increased during the next 18 hours; this is in line with the attribution of different effects to TPA depending on the length of treatment.Indeed, despite the strong activation of protein kinase C by short-term TPA treatment, downregulation of this important kinase by sustained TPA treatment is well known.
We then sought to identify the function that hNanos1 might have in cancer cells.Using functional assays, we showed that hNanos1 expression impairs the function of E-cadherin in cell-cell adhesion.Levels and extracellular exposure of E-cadherin protein were not changed on hNanos1 induction in the epithelial cells analyzed, indicating that hNanos1 interferes with the functionality of E-cadherin rather than with its expression.Furthermore, hNanos1 expression promoted cell migration and invasion of epithelial cells in vitro.Together, these data indicate that hNanos1 has a function in cancer cells deficient in E-cadherin.
Intriguing in this respect is our discovery that hNanos1 binds the Armadillo protein p120ctn and triggers its translocation from cell-cell contacts to the cytoplasm.The hNanos1-p120ctn association is correlated with abolishment of the arborization phenotype induced in fibroblasts by overexpression of p120ctn (13,33).This extensive branching of cellular protrusions is known to result from p120ctn-induced reduction in Rho activity with concomitant activation of Rac1 and Cdc42.This indicates a link between hNanos1 and Rho GTPase signaling via cytoplasmic p120ctn.The underlying mechanisms deserve further investigation.Unfortunately, several attempts to convincingly show an effect of hNanos1 on the activity of small GTPases of the Rho family were unsuccessful because the doxycycline treatment itself had a strong negative effect on the levels of GTP-bound Rac and Rho (data not shown).Nonetheless, it is worth mentioning that cytoplasmic p120ctn has been observed in different types of tumors (43)(44)(45)(46).The cytoplasmic localization of hNanos1 induced in our epithelial tumor cell system supports the possibility that hNanos1 has a function in the migratory effects mediated by cytoplasmic p120ctn (7).It is tempting to speculate on a similar mechanism to explain the role of Nanos in particular normal cell types, such as in primordial germ cell migration (17) and neuronal dendrite morphogenesis (38), especially because we saw an obvious increase in cytoplasmic p120ctn on hNanos1 induction in the tumor cell system we analyzed.
The Armadillo protein p120ctn interacts with the NH 2 -terminal part of hNanos1, which is conserved only among mammals. 6The more broadly conserved COOH-terminal part of Nanos and Nanosrelated proteins, containing the (CCHC) 2 zinc finger domain, is thought to represent the ancestral function of Nanos proteins in germ line development and survival in various invertebrate and vertebrate species (47,48).One can speculate that other specialized functions of Nanos genes were generated later in evolution in weakly conserved domains (30,35,48).p120ctn is dispensable for cadherin stability and adherens junction assembly in Drosophila and Caenorhabditis elegans (49, 50), but it is critically required in mammals (reviewed in refs.7,8).The combination of these data supports the possibility that the p120ctn-hNanos1 interaction has an important function in mammals but not in evolutionarily distinct organisms, such as flies and worms.
In summary, our data show that hNanos1 is a downstream target gene of E-cadherin capable of interfering with the proadhesive and anti-invasive functions of E-cadherin and of interacting with Armadillo proteins including p120ctn.Further research should investigate the following: (i) how hNanos1 transcript levels are regulated; (ii) how it can interfere with E-cadherin functionality; (iii) how it activates directional cell migration and invasion; (iv) what the molecular and biological consequences are of its interaction with Armadillo proteins; and (v) whether hNanos1 is an appropriate molecular target for therapy of certain cancers in which the function of E-cadherin is defective.

Figure 1 .
Figure 1. Cloning and initial characterization of hNanos1 as an E-cadherin-suppressed transcript.A, schematic representation of the human Nanos1 cDNA.ALU, Alu repeat within the 3 ¶-UTR; ORF, open reading frame of 876 bp; ZFD, zinc finger domain.Arrows, two alternative poly(A) addition sites; bar, localization of the initially isolated SSH clone.B, Northern blot analysis of E-cadherin and hNanos1 mRNAs in MDA-MB-231 cells and in the E-cadherin reexpressing derivative cell line MDA2BE5.36.GAPDH hybridization was used as RNA loading control.The sizes of the alternative hNanos1 transcripts are indicated in nucleotide number.C, Clustal W alignments of the (CCHC) 2 zinc finger domains of Nanos-related proteins 1 to 3 in three mammalian species: Hs, Homo sapiens (ref.22; this report and our unpublished data); Mm, Mus musculus (20, 21); Rn, Rattus norvegicus (our unpublished data).Reverse and gray shadings depict residues that are identical or similar to the column consensus in at least 50% of the aligned sequences.Arrows, conserved cysteine and histidine residues of the (CCHC) 2 motif.D, expression patterns of hNanos1 and E-cadherin mRNAs in various human cell lines.Strong expression of hNanos1 mRNA was detected by Northern blotting only in E-cadherin-deficient cell lines.GAPDH hybridization served as the RNA loading control.

Figure 2 .
Figure 2. Modulation of E-cadherin mRNA levels in SW480 human colorectal cancer cells induces inverse changes in hNanos1 transcripts.Transcripts were analyzed by Northern blot using GAPDH hybridization as RNA loading control.Columns, mRNA expression levels obtained after quantification and normalization to GAPDH.hNanos1 and E-cadherin transcript levels were compared in (A) subconfluent SW480 cells treated with TPA (250 ng/mL) for the indicated durations; (B) mock-transfected SW480 clones (K5 and K6 ) and Smad4-transfected clones (D1 and D14 ; ref. 24); (C) control K5 clone and Smad4-transfected D1 clone of SW480 cells that were either grown to confluence (C) or as a dense multilayer (M ).

Figure 3 .
Figure 3. Inducible expression of hNanos1 interferes with E-cadherin functionality in epithelial cells.A, immunoblotting using an anti-Myc antibody shows the presence of Myc-tagged hNanos1 in doxycycline-induced DLD1-Teton-hNanos1 cell clones, but not in doxycycline-treated DLD1-Teton-mock clones.B, expression of hNanos1 in doxycycline-treated DLD1-Teton-hNanos1 cells induces a clear morphotypic switch.C, immunoblotting analysis of protein levels expressed in DLD1-Teton-mock and DLD1-Teton-hNanos1 cells before (Àdox ) or after (+dox) induction of Myc-tagged hNanos1 protein.The levels of GAPDH served as loading controls.D, aggregation assays of DLD1-Teton-mock and DLD1-Teton-hNanos1 cells.Particle size distribution curves are shown for cells that were either induced (+dox ) or not (Àdox ) for 48 hours before the assay, in the presence or absence of an E-cadherin-blocking antibody (+DECMA-1 or ÀDECMA-1 ) during the assay.The starting population is indicated as N0.Aggregation after a 30-minute incubation (N30 ) was always reversed by DECMA-1 (y and E).Whereas both induced and noninduced mock cells formed large aggregates (n and .; left ), hNanos1-induction (+dox ) abrogated aggregation of DLD1-Teton-hNanos1 cells (n vs .; right ).E, immunofluorescence analysis of the cadherin/catenin complex in DLD1-Teton-hNanos1 cells before and after induction of Myc-tagged hNanos1 protein.Antigens detected are as indicated.Nuclei were counterstained with DAPI.

Figure 4 .
Figure 4. hNanos1 induces directional migration of tumor cells on a two-dimensional substrate and invasion into three-dimensional collagen type I. A, phase-contrast micrographs of the outgrowth periphery of DLD1-Teton-mock and DLD1-Teton-hNanos1 cells, either left untreated (ÀDox ) or treated with doxycycline (+Dox ) at t = 0 (a-d ) and t = 1 hour (e-h).Bar, 11 Am.B, the migration trajectories of the cells were computed from images recorded every 15 minutes for 1 hour.Trajectories are shown for the noninduced (ÀDox ) and induced (+Dox ) DLD-1-Teton-mock and DLD1-Teton-hNanos1 cells.Bar, 11 Am.C, migration speeds of DLD1-Teton-mock and DLD1-Teton-hNanos1 cells, either left untreated or treated with doxycycline.Columns, mean of three different experiments; bars, SD. **, P < 0.01.D, induction of hNanos1 results in invasion of DLD1-Teton-hNanos1 cells into type I collagen gels (filled columns ).White columns, data for DLD1-Teton-mock cells.Treatment with the E-cadherin-blocking antibody DECMA-1 was included as a positive control, resulting in induction of invasion in all conditions.

Figure 5 .
Figure5.The NH 2 -terminal domain of hNanos1 associates with the p120ctn Armadillo protein, and hNanos1 expression abolishes p120ctn-induced arborization.A, coimmunoprecipitation experiments on lysates of the indicated DLD1-Teton cell lines, treated or not treated with doxycycline for 48 hours, show association of Myc-tagged hNanos1 with p120ctn, but not with a-catenin.B, reciprocal coimmunoprecipitation on lysates of HEK293T cells, transiently cotransfected with a human p120ctn (isoform 3A) construct and a hNanos1 construct, encoding either hNanos1 full-length (FL ), its COOH-terminal zinc finger domain (ZnF ), or its NH 2 -terminal domain (N-term ).p120ctn associates with the full-length hNanos1 and with its NH 2 -terminal domain.C, transient cotransfection of NIH 3T3 fibroblast cells with 0.5 Ag of pCS3 backbone vector in combination with 0.25 Ag of a construct expressing EGFP-p120ctn fusion protein does not interfere with p120ctn-induced arborization (a; GFP fluorescence).In contrast, cotransfection with 0.5 Ag of a pCS3-hNanos1 construct, encoding either full-length hNanos1 or its NH 2 -terminal domain, abolishes formation of the dendrite-like extensions (b and c ; GFP fluorescence).Cells expressing ectopic hNanos1 were visualized by immunostaining with anti-Myc-tag antibody (d-f ).