Supplementary Figure 1 and Table 1 from CXCL13 Is Highly Produced by Sézary Cells and Enhances Their Migratory Ability via a Synergistic Mechanism Involving CCL19 and CCL21 Chemokines

Picchio, Maria Cristina; Scala, Enrico; Pomponi, Debora; Caprini, Elisabetta; Frontani, Marina; Angelucci, Irene; Mangoni, Antonella; Lazzeri, Cristina; Perez, Marie; Remotti, Daniele; Bonoldi, Emanuela; Benucci, Roberto; Baliva, Giannandrea; Lombardo, Giuseppe Alfonso; Napolitano, Monica; Russo, Giandomenico; Narducci, Maria Grazia

doi:10.1158/0008-5472.22376088.v1

00085472can080602-sup-sfig_1_and_table_1.pdf (81.9 kB)

Supplementary Figure 1 and Table 1 from CXCL13 Is Highly Produced by Sézary Cells and Enhances Their Migratory Ability via a Synergistic Mechanism Involving CCL19 and CCL21 Chemokines

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posted on 2023-03-30, 18:24 authored by Maria Cristina Picchio, Enrico Scala, Debora Pomponi, Elisabetta Caprini, Marina Frontani, Irene Angelucci, Antonella Mangoni, Cristina Lazzeri, Marie Perez, Daniele Remotti, Emanuela Bonoldi, Roberto Benucci, Giannandrea Baliva, Giuseppe Alfonso Lombardo, Monica Napolitano, Giandomenico Russo, Maria Grazia Narducci

Supplementary Figure 1 and Table 1 from CXCL13 Is Highly Produced by Sézary Cells and Enhances Their Migratory Ability via a Synergistic Mechanism Involving CCL19 and CCL21 Chemokines

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ARTICLE ABSTRACT

Chemokine and chemokine receptors expressed by normal and neoplastic lymphocytes play a key role in cell recruitment into skin and lymph nodes. The aim of this study was to get further insights into the role of chemokines in pathogenesis and progression of cutaneous T-cell lymphoma (CTCL) with particular regard to Sézary Syndrome (SS), a CTCL variant with blood involvement. Here, we show that functional CXCL13 homeostatic chemokine is strongly up-regulated in SS cells, well-detectable in skin lesions and lymph nodes, and measurable at high concentration in plasma of SS patients, at different levels during disease progression. Furthermore, we show that the addition of CXCL13 to CCL19 or to CCL21, the selective CCR7 agonists responsible for lymph node homing, strongly enhances the migration of CCR7+ SS cells. We also show that neutralization of the CCR7 receptor strongly impairs CCL19/21-induced chemotaxis of SS cells both in the absence or presence of CXCL13. Additional experiments performed to investigate the survival, adhesion, and metalloproteases secretion indicate that CXCL13 combined with CCL19 and CCL21 mainly affects the chemotaxis of SS cells. Our findings suggest that this newly described CXCL13 expression in SS represents a new pathogenetic mechanism of diagnostic significance. [Cancer Res 2008;68(17):7137–46]

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Biological Agents & Therapies Antireceptors Carcinogenesis Tumor initiation and promotion Hematological Cancers Leukemias Immunology Cytokines Progression, Invasion & Metastasis Migration and invasion Tumor progression

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Supplementary Figure 1 and Table 1 from CXCL13 Is Highly Produced by Sézary Cells and Enhances Their Migratory Ability via a Synergistic Mechanism Involving CCL19 and CCL21 Chemokines

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ARTICLE ABSTRACT

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