Supplementary Figure 12 from A Useful Approach to Identify Novel Small-Molecule Inhibitors of Wnt-Dependent Transcription

Ewan, Kenneth; Pająk, Bożena; Stubbs, Mark; Todd, Helen; Barbeau, Olivier; Quevedo, Camilo; Botfield, Hannah; Young, Rodrigo; Ruddle, Ruth; Samuel, Lee; Battersby, Alysia; Raynaud, Florence; Allen, Nicholas; Wilson, Stephen; Latinkic, Branko; Workman, Paul; McDonald, Edward; Blagg, Julian; Aherne, Wynne; Dale, Trevor

doi:10.1158/0008-5472.22381817.v1

00085472can101028-sup-fig12new.pdf (123.83 kB)

Supplementary Figure 12 from A Useful Approach to Identify Novel Small-Molecule Inhibitors of Wnt-Dependent Transcription

journal contribution

posted on 2023-03-30, 19:21 authored by Kenneth Ewan, Bożena Pająk, Mark Stubbs, Helen Todd, Olivier Barbeau, Camilo Quevedo, Hannah Botfield, Rodrigo Young, Ruth Ruddle, Lee Samuel, Alysia Battersby, Florence Raynaud, Nicholas Allen, Stephen Wilson, Branko Latinkic, Paul Workman, Edward McDonald, Julian Blagg, Wynne Aherne, Trevor Dale

Supplementary Figure 12 from A Useful Approach to Identify Novel Small-Molecule Inhibitors of Wnt-Dependent Transcription

History

ARTICLE ABSTRACT

The Wnt signaling pathway is frequently deregulated in cancer due to mutations in genes encoding APC, β-catenin, and axin. To identify small-molecule inhibitors of Wnt signaling as potential therapeutics, a diverse chemical library was screened using a transcription factor reporter cell line in which the activity of the pathway was induced at the level of Disheveled protein. A series of deconvolution studies was used to focus on three compound series that selectively killed cancer cell lines with constitutive Wnt signaling. Activities of the compounds included the ability to induce degradation of β-catenin that had been stabilized by a glycogen synthase kinase-3 (GSK-3) inhibitor. This screen illustrates a practical approach to identify small-molecule inhibitors of Wnt signaling that can seed the development of agents suitable to treat patients with Wnt-dependent tumors. Cancer Res; 70(14); 5963–73. ©2010 AACR.