journal contribution
posted on 2023-03-31, 22:07 authored by Cristina Carrato, Francesc Alameda, Anna Esteve-Codina, Estela Pineda, Oriol Arpí, Maria Martinez-García, Mar Mallo, Marta Gut, Raquel Lopez-Martos, Sonia Del Barco, Teresa Ribalta, Jaume Capellades, Josep Puig, Oscar Gallego, Carlos Mesia, Ana M Muñoz-Marmol, Ivan Archilla, Montserrat Arumí, Julie Marie Blanc, Beatriz Bellosillo, Silvia Menendez, Anna Esteve, Silvia Bagué, Ainhoa Hernandez, Jordi Craven-Bartle, Rafael Fuentes, Noemí Vidal, Iban Aldecoa, Nuria de la Iglesia, Carmen Balana Representative images comparing high versus low immunohistochemical expression of (A,B) EGFR,(C,D) PTEN, (E,F) SOX-2, (G,H) SHC1, (I,J) TCIRG1,(K,L) IDH1R132H (K:positive, L: negative), (M,N) Olig2,and (O,P) Ki67
Funding
Fundació la Marató de TV3
ISCIII
MINECO
Generalitat de Catalunya
AES
History
ARTICLE ABSTRACT
Molecular subtype classifications in glioblastoma may detect therapy sensitivities. IHC would potentially allow the identification of molecular subtypes in routine clinical practice.
Formalin-fixed, paraffin-embedded tumor samples of 124 uniformly treated, newly diagnosed patients with glioblastoma were submitted to RNA sequencing, IHC, and immune-phenotyping to identify differences in molecular subtypes associated with treatment sensitivities.
We detected high molecular and IHC overlapping of the The Cancer Genome Atlas (TCGA) mesenchymal subtype with instrinsic glioma subtypes (IGS) cluster 23 and of the TCGA classical subtype with IGS cluster 18. IHC patterns, gene fusion profiles, and immune-phenotypes varied across subtypes. IHC revealed that the TCGA classical subtype was identified by high expression of EGFR and low expression of PTEN, while the mesenchymal subtype was identified by low expression of SOX2 and high expression of two antibodies, SHC1 and TCIRG1, selected on the basis of RNA differential transcriptomic expression. The proneural subtype was identified by frequent positive IDH1 expression and high Olig2 and Ki67 expression. Immune-phenotyping showed that mesenchymal and IGS 23 tumors exhibited a higher positive effector cell score, a higher negative suppressor cell score, and lower levels of immune checkpoint molecules. The cell-type deconvolution analysis revealed that these tumors are highly enriched in M2 macrophages, resting memory CD4+ T cells, and activated dendritic cells, indicating that they may be ideal candidates for immunotherapy, especially with anti-M2 and/or dendritic cell vaccination.
There is a subset of tumors, frequently classified as mesenchymal or IGS cluster 23, that may be identified with IHC and could well be optimal candidates for immunotherapy.
