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Supplementary Fig. 7 from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

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posted on 2023-03-31, 01:05 authored by Susanne Kossatz, Brandon Carney, Melanie Schweitzer, Giuseppe Carlucci, Vesselin Z. Miloushev, Uday B. Maachani, Prajwal Rajappa, Kayvan R. Keshari, David Pisapia, Wolfgang A. Weber, Mark M. Souweidane, Thomas Reiner

Monitoring tumor growth using [18F]PARPi. Display of weekly imaging of tumor development in adult ntv-a;p53fl/fl mice at the indicated time after DF1 cell injection. PET/CT imaging was always conducted 2 h p.i. of [18F]PARPi. Animals were sacrificed after week 6 and tumor presence was confirmed histologically. (b) One animal showed no increase in [18F]PARPi uptake over the weeks and we confirmed histologically that tumor development was absent in this case.

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National Institutes of Health

National Science Foundation's Integrative Graduate Education and Research Traineeship

German Research Foundation

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ARTICLE ABSTRACT

Diffuse intrinsic pontine glioma (DIPG) is a childhood brainstem tumor with a universally poor prognosis. Here, we characterize a positron emission tomography (PET) probe for imaging DIPG in vivo. In human histological tissues, the probes target, PARP1, was highly expressed in DIPG compared to normal brain. PET imaging allowed for the sensitive detection of DIPG in a genetically engineered mouse model, and probe uptake correlated to histologically determined tumor infiltration. Imaging with the sister fluorescence agent revealed that uptake was confined to proliferating, PARP1-expressing cells. Comparison with other imaging technologies revealed remarkable accuracy of our biomarker approach. We subsequently demonstrated that serial imaging of DIPG in mouse models enables monitoring of tumor growth, as shown in modeling of tumor progression. Overall, this validated method for quantifying DIPG burden would serve useful in monitoring treatment response in early phase clinical trials. Cancer Res; 77(8); 2112–23. ©2017 AACR.

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    Cancer Research

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    Keywords

    BiomarkersCarcinogen exposure biomarkersCarcinogenesisDNA damage and repairCns CancersImagingPositron emission tomography (PET)Small animal imagingPreclinical ModelsAnimal models of cancerProgression, Invasion & MetastasisImaging of tumor progression and metastasis

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