Supplementary Experimental Procedures from Neoantigen Presentation and IFNγ Signaling on the Same Tumor-associated Macrophage are Necessary for CD4 T Cell–mediated Antitumor Activity in Mice

Perez-Diez, Ainhoa; Liu, Xiangdong; Matzinger, Polly

doi:10.1158/2767-9764.22544700.v1

Supplementary Experimental Procedures from Neoantigen Presentation and IFNγ Signaling on the Same Tumor-associated Macrophage are Necessary for CD4 T Cell–mediated Antitumor Activity in Mice

journal contribution

posted on 2023-04-04, 01:25 authored by Ainhoa Perez-Diez, Xiangdong Liu, Polly Matzinger

Supplementary Materials and Methods

Funding

Division of Intramural Research, National Institute of Allergy and Infectious Diseases (DIR, NIAID)

History

ARTICLE ABSTRACT

In the tumor microenvironment, TAMs are capable of presenting tumor antigens to effector CD4 T cells. Upon antigen recognition, the CD4 cells transform transcriptionally, phenotypically, and functionally the TAMs inducing tumor rejection. This reeducation process requires both cognate interaction and IFNγ signaling on the same macrophage.