Supplementary Data from The Pro-Oncogenic Adaptor CIN85 Acts as an Inhibitory Binding Partner of Hypoxia-Inducible Factor Prolyl Hydroxylase 2
journal contribution
posted on 2023-03-31, 02:47 authored by Nina Kozlova, Daniela Mennerich, Anatoly Samoylenko, Elitsa Y. Dimova, Peppi Koivunen, Ekaterina Biterova, Kati Richter, Antti Hassinen, Sakari Kellokumpu, Aki Manninen, Ilkka Miinalainen, Virpi Glumoff, Lloyd Ruddock, Lyudmyla Borysivna Drobot, Thomas Kietzmann<p>Supplementary figure 1. CIN85 interacts with PHD2 via three N-terminal SH3 domains. Supplementary figure 2. The amino acids 81-98 within the N-terminal region of PHD2 have a major impact on the interaction with CIN85. Supplementary figure 3. CRISPR/Cas9-mediated generation of MDA-MB-231 cells lacking the amino acids critical for interaction with CIN85. Supplementary figure 4. Loss of PHD2-CIN85 interaction does not influence HIF-1α and HIF-2α mRNA levels. Supplementary figure 5. Lack of the CIN85-PHD2 interaction in E10 and E12 cells mediates HIF-α resistance upon depletion of CIN85. Supplementary figure 6. Microscopy images of S, E10, and E12 cells. Supplementary figure 7. Live cell proliferation analysis of S, E10, and E12 cells. Supplementary figure 8. CRISPR/Cas9 mediated EGLN1 (PHD2) editing in MDA-MB-231 cells with EGLN1-sgRNA-219 and with EGLN1-sgRNA-292 did not show off-target effects.</p>
