Supplemental Methods, Figure Legends, Tables 1 - 5 from EGF Receptor Promotes Prostate Cancer Bone Metastasis by Downregulating miR-1 and Activating TWIST1

Chang, Yung-Sheng; Chen, Wei-Yu; Yin, Juan Juan; Sheppard-Tillman, Heather; Huang, Jiaoti; Liu, Yen-Nien

doi:10.1158/0008-5472.22406150.v1

Supplemental Methods, Figure Legends, Tables 1 - 5 from EGF Receptor Promotes Prostate Cancer Bone Metastasis by Downregulating miR-1 and Activating TWIST1

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posted on 2023-03-30, 23:11 authored by Yung-Sheng Chang, Wei-Yu Chen, Juan Juan Yin, Heather Sheppard-Tillman, Jiaoti Huang, Yen-Nien Liu

<p>Table S1. Positions of AT-rich minimal consensus sequences (ATRSs). Table S2. Primer sequences of the 3'UTR and promoter reporter constructs. Table S3. Primer sequences for the qRT-PCR. Table S4. Antibody information for Western blotting. Table S5. Antibody information and primer sequences of the ChIP assay.</p>

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DOI - Is supplement to EGF Receptor Promotes Prostate Cancer Bone Metastasis by Downregulating miR-1 and Activating TWIST1

ARTICLE ABSTRACT

Dysregulation of the EGFR signaling axis enhances bone metastases in many solid cancers. However, the relevant downstream effector signals in this axis are unclear. miR-1 was recently shown to function as a tumor suppressor in prostate cancer cells, where its expression correlated with reduced metastatic potential. In this study, we demonstrated a role for EGFR translocation in regulating transcription of miR-1-1, which directly targets expression of TWIST1. Consistent with these findings, we observed decreased miR-1 levels that correlated with enhanced expression of activated EGFR and TWIST1 in a cohort of human prostate cancer specimens and additional datasets. Our findings support a model in which nuclear EGFR acts as a transcriptional repressor to constrain the tumor-suppressive role of miR-1 and sustain oncogenic activation of TWIST1, thereby leading to accelerated bone metastasis. Cancer Res; 75(15); 3077–86. ©2015 AACR.

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Biomarkers Carcinogenesis Signal transduction Gene Regulation Inducible gene expression Genitourinary Cancers Prostate cancer Oncogenes & Tumor Suppressors

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Supplemental Methods, Figure Legends, Tables 1 - 5 from EGF Receptor Promotes Prostate Cancer Bone Metastasis by Downregulating miR-1 and Activating TWIST1

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