American Association for Cancer Research
00085472can170330-sup-178436_2_supp_4129932_6s9n6k.pdf (1.16 MB)

Supplemental Materials from Integrating DNA Methylation and Hydroxymethylation Data with the Mint Pipeline

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journal contribution
posted on 2023-03-31, 01:40 authored by Raymond G. Cavalcante, Snehal Patil, Yongseok Park, Laura S. Rozek, Maureen A. Sartor

Supplementary Tables 1-3 and Supplementary Figures 1-5



National Institute of General Medical Sciences



DNA methylation (5mC) plays important roles in mammalian development, oncogenesis, treatment response, and responses to the environment. DNA hydroxymethylation (5hmC) is also an informative epigenetic mark with distinct roles in regulation and cancer. Gold-standard, widely used technologies (bisulfite conversion, followed by deep sequencing) cannot distinguish between 5mC and 5hmC. Therefore, additional experiments are required to differentiate the two marks, and in silico methods are needed to analyze, integrate, and interpret these data. We developed the Methylation INTegration (mint) pipeline to support the comprehensive analysis of bisulfite conversion and immunoprecipitation-based methylation and hydroxymethylation assays, with additional steps toward integration, visualization, and interpretation. The pipeline is available as both a command line and a Galaxy graphical user interface tool. Both implementations require minimal configuration while remaining flexible to experiment specific needs. Cancer Res; 77(21); e27–30. ©2017 AACR.