American Association for Cancer Research
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Supplemental Figures and Tables from Deficiency in Protein Tyrosine Phosphatase PTP1B Shortens Lifespan and Leads to Development of Acute Leukemia

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journal contribution
posted on 2023-03-31, 01:21 authored by Samantha Le Sommer, Nicola Morrice, Martina Pesaresi, Dawn Thompson, Mark A. Vickers, Graeme I. Murray, Nimesh Mody, Benjamin G. Neel, Kendra K. Bence, Heather M. Wilson, Mirela Delibegović

Supplemental Figure 1 (SF1) describes lifespan in global PTP1B-/- mice. SF2 shows development of leukemia is associated with myeloid PTP1B complete deficiency. SF3 shows tumors in LysM PTP1B-/- mice. SF4 presents LysM PTP1B-/- microarray data. SF5 presents STAT5 expression data. SF6 presents additional STAT3 ChIP seq data from LysM PTP1B mice. Tables 1-4 demonstrate detailed statistics for survival data on LysM PTP1B and global PTP1B-/- mice as well as hepatic pre-tumour microarray data from LysM PTP1B-/- and control mice.


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University of Aberdeen Institute of Medical Sciences



Protein tyrosine phosphatase PTP1B is a critical regulator of signaling pathways controlling metabolic homeostasis, cell proliferation, and immunity. In this study, we report that global or myeloid-specific deficiency of PTP1B in mice decreases lifespan. We demonstrate that myeloid-specific deficiency of PTP1B is sufficient to promote the development of acute myeloid leukemia. LysM-PTP1B−/− mice lacking PTP1B in the innate myeloid cell lineage displayed a dysregulation of bone marrow cells with a rapid decline in population at midlife and a concomitant increase in peripheral blood blast cells. This phenotype manifested further with extramedullary tumors, hepatic macrophage infiltration, and metabolic reprogramming, suggesting increased hepatic lipid metabolism prior to overt tumor development. Mechanistic investigations revealed an increase in anti-inflammatory M2 macrophage responses in liver and spleen, as associated with increased expression of arginase I and the cytokines IL10 and IL4. We also documented STAT3 hypersphosphorylation and signaling along with JAK-dependent upregulation of antiapoptotic proteins Bcl2 and BclXL. Our results establish a tumor suppressor role for PTP1B in the myeloid lineage cells, with evidence that its genetic inactivation in mice is sufficient to drive acute myeloid leukemia.Significance: This study defines a tumor suppressor function for the protein tyrosine phosphatase PTP1B in myeloid lineage cells, with evidence that its genetic inactivation in mice is sufficient to drive acute myeloid leukemia. Cancer Res; 78(1); 75–87. ©2017 AACR.