15357163mct150765-sup-154954_1_supp_0_r5bjrr.docx (41.28 kB)
Supplemental Figure Legends from NQO1-Mediated Tumor-Selective Lethality and Radiosensitization for Head and Neck Cancer
journal contributionposted on 2023-04-03, 15:41 authored by Long-Shan Li, Srilakshmi Reddy, Zhen-Hua Lin, Shuangping Liu, Hyunsil Park, Stephen G. Chun, William G. Bornmann, Joel Thibodeaux, Jingsheng Yan, Gaurab Chakrabarti, Xian-Jin Xie, Baran D. Sumer, David A. Boothman, John S. Yordy
Supplemental Figure Legends; Supplemental Table Legends
UT Southwestern Department of Radiation Oncology
ARTICLE ABSTRACTIonizing radiation (IR) is a key therapeutic regimen for many head and neck cancers (HNC). However, the 5-year overall survival rate for locally advanced HNCs is approximately 50% and better therapeutic efficacy is needed. NAD(P)H:quinone oxidoreductase 1 (NQO1) is overexpressed in many cancers, and β-lapachone (β-lap), a unique NQO1 bioactivatable drug, exploits this enzyme to release massive reactive oxygen species (ROS) that synergize with IR to kill by programmed necrosis. β-Lap represents a novel therapeutic opportunity in HNC leading to tumor-selective lethality that will enhance the efficacy of IR. Immunohistochemical staining and Western blot assays were used to assess the expression levels of NQO1 in HNC cells and tumors. Forty-five percent of endogenous HNCs expressed elevated NQO1 levels. In addition, multiple HNC cell lines and tumors demonstrated elevated levels of NQO1 expression and activity and were tested for anticancer lethality and radiosensitization by β-lap using long-term survival assays. The combination of nontoxic β-lap doses and IR significantly enhanced NQO1-dependent tumor cell lethality, increased ROS, TUNEL-positive cells, DNA damage, NAD+, and ATP consumption, and resulted in significant antitumor efficacy and prolonged survival in two xenograft murine HNC models, demonstrating β-lap radiosensitization of HNCs through a NQO1-dependent mechanism. This translational study offers a potential biomarker-driven strategy using NQO1 expression to select tumors susceptible to β-lap–induced radiosensitization. Mol Cancer Ther; 15(7); 1757–67. ©2016 AACR.
CarcinogenesisAnimal models of carcinogenesisChemotherapyBiochemical modulators of the therapeutic indexDrug MechanismsModulation of DNA repairHead, Neck & Oral CancersRadiation OncologyCombined modality therapiesModification of radiation sensitivityMolecular targets of radiation responseRadioprotectors and radiosensitizersSmall Molecule Agents