Supp. Figure 5 from Targeted AKT Inhibition in Prostate Cancer Cells and Spheroids Reduces Aerobic Glycolysis and Generation of Hyperpolarized [1-¹³C] Lactate

Hyperpolarized MRS of sodium alginate thread cultures. (A) Bright-field image of an alginate thread in culture and (B) H&E stain of the same thread and (C) another cross-section stained with Ki-67. (D) Representative images of an alginate thread cultured in a rotating plate over a period of 4 days and stained with Ki67 All scale bars represent 500 μm. (E) Thread cultures wound on a custom-printed 3D insert, directly deposited into a 5mm NMR tube metabolizes hyperpolarized [1-13C] pyruvate to lactate after injection of 1mM hyperpolarized pyruvate (F). Quantification of integrals of the lactate and pyruvate hydrate peaks over time (G).