Figure S5 from Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

Lambros, Maryou B.; Seed, George; Sumanasuriya, Semini; Gil, Veronica; Crespo, Mateus; Fontes, Mariane; Chandler, Rob; Mehra, Niven; Fowler, Gemma; Ebbs, Berni; Flohr, Penny; Miranda, Susana; Yuan, Wei; Mackay, Alan; Ferreira, Ana; Pereira, Rita; Bertan, Claudia; Figueiredo, Ines; Riisnaes, Ruth; Rodrigues, Daniel Nava; Sharp, Adam; Goodall, Jane; Boysen, Gunther; Carreira, Suzanne; Bianchini, Diletta; Rescigno, Pasquale; Zafeiriou, Zafeiris; Hunt, Joanne; Moloney, Deirdre; Hamilton, Lucy; Neves, Rui P.; Swennenhuis, Joost; Andree, Kiki; Stoecklein, Nikolas H.; Terstappen, Leon W.M.M.; de Bono, Johann S.

doi:10.1158/1078-0432.22469396.v1

Figure S5 from Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

https://doi.org/10.1158/1078-0432.22469396

journal contribution

posted on 2023-03-31, 20:41 authored by Maryou B. Lambros, George Seed, Semini Sumanasuriya, Veronica Gil, Mateus Crespo, Mariane Fontes, Rob Chandler, Niven Mehra, Gemma Fowler, Berni Ebbs, Penny Flohr, Susana Miranda, Wei Yuan, Alan Mackay, Ana Ferreira, Rita Pereira, Claudia Bertan, Ines Figueiredo, Ruth Riisnaes, Daniel Nava Rodrigues, Adam Sharp, Jane Goodall, Gunther Boysen, Suzanne Carreira, Diletta Bianchini, Pasquale Rescigno, Zafeiris Zafeiriou, Joanne Hunt, Deirdre Moloney, Lucy Hamilton, Rui P. Neves, Joost Swennenhuis, Kiki Andree, Nikolas H. Stoecklein, Leon W.M.M. Terstappen, Johann S. de Bono

<p>Organoid cultures of CTCs acquired by apheresis from patient P05: a) Dendrogram and heat map of hierarchical clustering, based on Euclidean distance, for patient P05 evaluating CTC (green bars) and organoid CNAs (red). b) Micrographs of two organoids from P05 with scale bar in bottom left (100Âµm). c) Phylogenetic tree showing the cultured organoids have CNA that cluster with CTCs. d) Two organoids and 3 CTCs with truncal CNA including shared BRCA2 loss and AR amplification but sub-clonal chromosome 1 aberrations.</p>

Funding

CTC-TRAP:EUFP7

Movember/PCUK

Prostate Cancer Foundation

Prostate Cancer UK

Cancer–Prostate Cancer Foundation Prostate Dream Team Translational Cancer Research

PCF

Cancer Research UK

History

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DOI - Is supplement to Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

ARTICLE ABSTRACT

Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635–44. ©2018 AACR.

Figure S5 from Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

Funding

CTC-TRAP:EUFP7

Movember/PCUK

Prostate Cancer Foundation

Prostate Cancer UK

Cancer–Prostate Cancer Foundation Prostate Dream Team Translational Cancer Research

PCF

Cancer Research UK

History

Related Materials

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