American Association for Cancer Research
Browse

Figure S4 from Kindlin-1 Promotes Pulmonary Breast Cancer Metastasis

Download (502.44 kB)
journal contribution
posted on 2023-03-31, 01:24 authored by Sana Sarvi, Hitesh Patel, Jun Li, Georgia L. Dodd, Helen Creedon, Morwenna Muir, Jocelyn Ward, John C. Dawson, Martin Lee, Jayne Culley, Donald M. Salter, Andrew H. Sims, Adam Byron, Valerie G. Brunton

Figure S4 shows supporting data that Kindlin-1 reduces integrin activation and lung colonization

Funding

Cancer Research UK

European Research Council

History

ARTICLE ABSTRACT

In breast cancer, increased expression of the cytoskeletal adaptor protein Kindlin-1 has been linked to increased risks of lung metastasis, but the functional basis is unknown. Here, we show that in a mouse model of polyomavirus middle T antigen–induced mammary tumorigenesis, loss of Kindlin-1 reduced early pulmonary arrest and later development of lung metastasis. This phenotype relied on the ability of Kindlin-1 to bind and activate β integrin heterodimers. Kindlin-1 loss reduced α4 integrin–mediated adhesion of mammary tumor cells to the adhesion molecule VCAM-1 on endothelial cells. Treating mice with an anti–VCAM-1 blocking antibody prevented early pulmonary arrest. Kindlin-1 loss also resulted in reduced secretion of several factors linked to metastatic spread, including the lung metastasis regulator tenascin-C, showing that Kindlin-1 regulated metastatic dissemination by an additional mechanism in the tumor microenvironment. Overall, our results show that Kindlin-1 contributes functionally to early pulmonary metastasis of breast cancer.Significance: These findings provide a mechanistic proof in mice that Kindin-1, an integrin-binding adaptor protein, is a critical mediator of early lung metastasis of breast cancer. Cancer Res; 78(6); 1484–96. ©2018 AACR.

Usage metrics

    Cancer Research

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC