journal contribution
posted on 2023-03-31, 03:27 authored by Siqi Wu, Liyuan Zhang, Jieqiong Deng, Binbin Guo, Fang Li, Yirong Wang, Rui Wu, Shenghua Zhang, Jiachun Lu, Yifeng Zhou <p>Fig. S4 YY1 and AR jointly regulate the expression of eEF2K and in turn affect proliferation and apoptosis of ESCC cells under acute ND.</p>
Funding
National Scientific Foundation of China
Science Foundation for Distinguished Young Scholars in Jiangsu
Jiangsu Higher Education Institutions
National Key R&D Program of China
Program for Guangdong Introducing Innovative and Entrepreneurial Teams
History
ARTICLE ABSTRACT
Long noncoding RNAs (lncRNA) have been shown to play critical roles in many diseases, including esophageal squamous cell carcinoma (ESCC). Recent studies have reported that some lncRNA encode functional micropeptides. However, the association between ESCC and micropeptides encoded by lncRNA remains largely unknown. In this study, we characterized a Y-linked lncRNA, LINC00278, which was downregulated in male ESCC. LINC00278 encoded a Yin Yang 1 (YY1)-binding micropeptide, designated YY1BM. YY1BM was involved in the ESCC progression and inhibited the interaction between YY1 and androgen receptor (AR), which in turn decreased expression of eEF2K through the AR signaling pathway. Downregulation of YY1BM significantly upregulated eEF2K expression and inhibited apoptosis, thus conferring ESCC cells more adaptive to nutrient deprivation. Cigarette smoking decreased m6A modification of LINC00278 and YY1BM translation. In conclusion, these results provide a novel mechanistic link between cigarette smoking and AR signaling in male ESCC progression.
Posttranscriptional modification of a micropeptide-encoding lncRNA is negatively impacted by cigarette smoking, disrupting negative regulation of the AR signaling pathway in male ESCC.
