Figure S2. BET protein specificity and proliferation/survival from BRD4 Regulates Breast Cancer Dissemination through Jagged1/Notch1 Signaling

(A-C) Relative mRNA expression levels of BRD2 (A), BRD3 (B), and BRD4 (C) in scrambled siRNA control or BRD2-, BRD3-, BRD4-depleted MDA-MB-231 cells. Bars show means (plus minus) SEM of three independent experiments. Statistical analyses were performed by using one-way ANOVA. (D) Dotplot of Annexin V/propidium iodide co-staining of MDA-MB-231 and SUM149PT cells treated with scramble, siBRD2, siBRD3 or BRD4 siRNA for 72h. Quadrant 1 (Q1): necrotic cells, Q2: late apoptotic cells, Q3: early apoptotic cells, Q4: viable cells. At least 20,000 cells were analyzed (n=3). (E) Cell cycle distribution of MDA-MB-231 and SUM149PT treated with scrambled siRNA control, siBRD2, siBRD3 or siBRD4 for 72h. At least 10,000 cells were analyzed (n=3). (F) MTT assay showing proliferation of MDA-MB-231, SUM149PT and MCF-7 treated with scramble, siBRD2, siBRD3 or BRD4 siRNA for 72h for the indicated times. Bars represent means (plus minus) SEM of three independent experiments. Statistical analysis was performed by using two-way ANOVA.