American Association for Cancer Research
10780432ccr173284-sup-191916_2_supp_4841331_p2n4p2.pdf (16.97 kB)

Figure S1 from Parallel Accumulation of Tumor Hyaluronan, Collagen, and Other Drivers of Tumor Progression

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journal contribution
posted on 2023-03-31, 20:47 authored by Xiaoming Li, H. Michael Shepard, Jessica A. Cowell, Chunmei Zhao, Ryan J. Osgood, Sanna Rosengren, Barbara Blouw, Sheryl A. Garrovillo, Mark D. Pagel, Clifford J. Whatcott, Haiyong Han, Daniel D. Von Hoff, Darin M. Taverna, Michael J. LaBarre, Daniel C. Maneval, Curtis B. Thompson

In HA-accumulating AsPC-1/HAS3 tumors, low-dose PEGPH20 decreased tumor HA levels by >64%; whereas the level of tumor HA in the already very HA-low parental AsPC-1 (AsPC-1-P) tumors remained statistically unchanged (n = 10/group).




National Foundation for Cancer Research



Purpose: The tumor microenvironment (TME) evolves to support tumor progression. One marker of more aggressive malignancy is hyaluronan (HA) accumulation. Here, we characterize biological and physical changes associated with HA-accumulating (HA-high) tumors.Experimental Design: We used immunohistochemistry, in vivo imaging of tumor pH, and microdialysis to characterize the TME of HA-high tumors, including tumor vascular structure, hypoxia, tumor perfusion by doxorubicin, pH, content of collagen. and smooth muscle actin (α-SMA). A novel method was developed to measure real-time tumor-associated soluble cytokines and growth factors. We also evaluated biopsies of murine and pancreatic cancer patients to investigate HA and collagen content, important contributors to drug resistance.Results: In immunodeficient and immunocompetent mice, increasing tumor HA content is accompanied by increasing collagen content, vascular collapse, hypoxia, and increased metastatic potential, as reflected by increased α-SMA. In vivo treatment of HA-high tumors with PEGylated recombinant human hyaluronidase (PEGPH20) dramatically reversed these changes and depleted stores of VEGF-A165, suggesting that PEGPH20 may also diminish the angiogenic potential of the TME. Finally, we observed in xenografts and in pancreatic cancer patients a coordinated increase in HA and collagen tumor content.Conclusions: The accumulation of HA in tumors is associated with high tIP, vascular collapse, hypoxia, and drug resistance. These findings may partially explain why more aggressive malignancy is observed in the HA-high phenotype. We have shown that degradation of HA by PEGPH20 partially reverses this phenotype and leads to depletion of tumor-associated VEGF-A165. These results encourage further clinical investigation of PEGPH20. Clin Cancer Res; 24(19); 4798–807. ©2018 AACR.

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