journal contribution
posted on 2023-12-15, 08:43 authored by Hirofumi Asano, Abdelazim Elsayed Elhelaly, Fuminori Hyodo, Ryota Iwasaki, Yoshifumi Noda, Hiroki Kato, Koki Ichihashi, Hiroyuki Tomita, Masaharu Murata, Takashi Mori, Masayuki Matsuo Sensitivity of in vivo deuterium MRI and kinetics of 2H enrichments in C26 xenograft-bearing BALB/c-nu mice allowed to drink D2O freely
(A) Representative coronal deuterium MRI images of the distribution of 2H in the body of C26 colorectal carcinoma xenograft-bearing mice during 10 days of D2O administration. Images were collected on days 1, 3, 7, and 10 of the first exposure to D2O. (B) Representative T1-weighted MRI images of mouse body parts. (C) Full-body MRI images after placing the MRI images of three body parts together show the distribution of 2H and 1H signals collected throughout the body. (D) Quantification of 2H MRI signals of body tissues. Analysis was performed using Image J software. The data show the means ± SD (n=5).
Funding
Japan Agency for Medical Research and Development (AMED)
Japan Society for the Promotion of Science (JSPS)
MEXT Quantum Leap Flagship Program (MEXT Q-LEAP)
History
ARTICLE ABSTRACT
An accurate and noninvasive assessment of tumor response following treatment other than traditional anatomical imaging techniques is essential. Deuterium magnetic resonance spectroscopic (MRS) imaging has been demonstrated as an alternative for cancer metabolic imaging by high-field MRI using deuterium-labeled molecules. The study aim was to use 2H tissue labeling and deuterium MRI at clinical field strength for tumor visualization and assessment of three anticancer therapies in pancreatic cancer model mice.
MIA PaCa-2 pancreatic carcinoma and C26 colorectal carcinoma models of BALB/c-nu mice was prepared, and repeated deuterium MRI was performed during the first 10 days of free drinking of 30% D2O to track 2H distribution in tissues. 2H accumulation in the tumor after irradiation, bevacizumab administration, or gemcitabine administration was also measured in MIA PaCa-2–bearing mice. Confirmatory proton MRI, ex vivo metabolic hyperpolarization 13C-MRS, and histopathology were performed.
The mouse's whole-body distribution of 2H was visible 1 day after drinking, and the signal intensity increased daily. Although the tumor size did not change 1 and 3 days after irradiation, the amount of 2H decreased significantly. The 2H image intensity of the tumor also significantly decreased after the administration of bevacizumab or gemcitabine. Metabolic hyperpolarization 13C-MRS, proton MRI, and 2H-NMR spectroscopy confirmed the efficacy of the anticancer treatments.
Deuterium MRI at 1.5T proved feasible to track 2H distribution throughout mouse tissues during D2O administration and revealed a higher 2H accumulation in the tumor xenografts. This research demonstrated a promising successful method for preliminary assessment of radiotherapy and chemotherapy of cancer.
