American Association for Cancer Research

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Figure S1-S9 from Lnc-C/EBPβ Negatively Regulates the Suppressive Function of Myeloid-Derived Suppressor Cells

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journal contribution
posted on 2023-04-03, 23:26 authored by Yunhuan Gao, Wei Sun, Wencong Shang, Yuanyuan Li, Dan Zhang, Tianze Wang, Xipeng Zhang, Shiwu Zhang, Yuan Zhang, Rongcun Yang

Figure S1. Characterization of inflammatory cytokine and tumor mediated MDSCs. Figure S2. Characteristics of lnc-C/EBPβ. Figure S3. Sequences of mouse lnc-C/EBPβ and humanlnc-C/EBPβ. Figure S4. The suppressive MDSC isolated from tumors may express high levels of both Lnc-C/EBPβ and C/EBPβ. Figure S5. The tumor associated factors mediated Lnc-C/EBP� are dependent on STAT3. Figure S6. Gene microarray of lnc-C/EBPβ shRNA/letiviruses and lnc-C/EBPβ/lentiviruses transduced cells. Figure S7. Lnc-C/EBPβ impedes differentiation of M-MDSCs. Figure S8. Lnc-C/EBPβ also impacts survival of MDSCs. Figure S9. The binding of Lnc-C/EBPβ to C/EBPβ LIP did not affect the transcriptional activity of C/EBPβ LAP-LAP* or LAP-LAP.



National Natural Science Foundation of China and the Israel Science Foundation

National Key Research and Development Program of China



Myeloid-derived suppressor cells (MDSC) are regulators of immune responses in cancer. The differentiation and function of these MDSCs may be regulated through multiple factors, such as microRNAs. However, the effect of long noncoding RNAs (lncRNA) on the differentiation and function of MDSCs is poorly understood. We identified a long noncoding RNA (lncRNA) named lnc-C/EBPβ in MDSCs, which may control suppressive functions of MDSCs. Lnc-C/EBPβ could be induced in in vitro and in vivo tumor and inflammatory environments. It regulated a set of target transcripts, such as Arg-1, NOS2, NOX2, and COX2, to control immune-suppressive function and differentiation of MDSCs. This lncRNA was also able to bind to the C/EBPβ isoform LIP to inhibit the activation of C/EBPβ. We also found that the conserved homologue lnc-C/EBPβ has a similar function to murine lnc-C/EBPβ. These findings suggest a negative feedback role for lnc-C/EBPβ in controlling the immunosuppressive functions of MDSC in the tumor environment. Cancer Immunol Res; 6(11); 1352–63. ©2018 AACR.

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