Figure S1-S7 from Skp2-Mediated Stabilization of MTH1 Promotes Survival of Melanoma Cells upon Oxidative Stress

Figure S1 shows the turnover rates of MTH1 mRNA remained similar between melanoma cells with relatively high (Mel-FH) and low (IgR3) expression of the transcript. Figure S2 shows silencing or overexpression of Skp2 did not alter the expression levels of the MTH1 transcript in melanoma cells. Figure S3 shows silencing of MTH1 did not impinge on the expression Skp2. Figure S4 shows Skp2-NES retains the ability to bind to MTH1. Figure S5 shows Skp2 silencing inhibits melanoma cell proliferation but does trigger apoptosis. Figure S6 shows the oxidative stress inducer elesclomol increases ROS levels. Figure S7 shows BRAF mutant melanoma cells selected for resistance to vemurafenib are more susceptible to killing by knockdown of Skp2 or MTH1.