journal contribution
posted on 2023-03-31, 01:45 authored by Qiheng Gou, Linbo Gao, Xinwen Nie, Wenchen Pu, Jingqiang Zhu, Yichao Wang, Xuesha Liu, Shuangyan Tan, Jian-Kang Zhou, Yanqiu Gong, Juan He, Ke Wu, Yuxin Xie, Wanjun Zhao, Lunzhi Dai, Lunxu Liu, Rong Xiang, Yu-Quan Wei, Lin Zhang, Yong Peng Supplementary figures and lncAB sequences
Funding
National Key R&D Program of China
National Natural Science Foundation of China
Special Research Foundation of Doctoral Priority to the Development of Field Project
Science and Technology Pillar Program of Sichuan Province
History
ARTICLE ABSTRACT
Long noncoding RNAs (lncRNA) are emerging as a novel class of regulators in gene expression associated with tumorigenesis. However, the role of lncRNAs in papillary thyroid carcinoma (PTC) is poorly understood. Here, we conducted global lncRNA profiling and identified lncRNA AB074169 (lncAB) as significantly downregulated in PTC. Decreased expression of lncAB in PTC was caused by CpG hypermethylation within its gene promoter. Functional studies showed that lncAB overexpression led to cell-cycle arrest and tumor growth inhibition in vitro and in vivo, whereas lncAB knockdown promoted cell proliferation. Mechanistic analyses revealed that lncAB bound KH-type splicing regulatory protein (KHSRP) and also decreased expression of KHSRP, thus increasing CDKN1a (p21) expression and decreasing CDK2 expression to repress cell proliferation. Taken together, these findings demonstrate that lncAB functions as a tumor suppressor during PTC tumorigenesis.Significance: These findings identify a tumor-suppressive long noncoding RNA in papillary thyroid carcinoma.Graphical Abstract:http://cancerres.aacrjournals.org/content/canres/78/15/4163/F1.large.jpg. Cancer Res; 78(15); 4163–74. ©2018 AACR.
