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10780432ccr182202-sup-205079_2_supp_5227998_pjvt6b.pptx (136.82 kB)

Supplementary figure 3 from Endogenous Production of IL1B by Breast Cancer Cells Drives Metastasis and Colonization of the Bone Microenvironment

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posted on 2023-03-31, 21:20 authored by Claudia Tulotta, Diane V. Lefley, Katy Freeman, Walter M. Gregory, Andrew M. Hanby, Paul R. Heath, Faith Nutter, J. Mark Wilkinson, Amy R. Spicer-Hadlington, Xinming Liu, Steven M.J. Bradbury, Lisa Hambley, Victoria Cookson, Gloria Allocca, Marianna Kruithof de Julio, Robert E. Coleman, Janet E. Brown, Ingunn Holen, Penelope D. Ottewell

Incubation with high concentrations of exogenous IL-1B modestly increases tumour cell migration and invasion.

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Medical Research Council

UK and Weston Park Cancer Charity

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ARTICLE ABSTRACT

Breast cancer bone metastases are incurable, highlighting the need for new therapeutic targets. After colonizing bone, breast cancer cells remain dormant, until signals from the microenvironment stimulate outgrowth into overt metastases. Here we show that endogenous production of IL1B by tumor cells drives metastasis and growth in bone. Tumor/stromal IL1B and IL1 receptor 1 (IL1R1) expression was assessed in patient samples and effects of the IL1R antagonist, Anakinra, or the IL1B antibody canakinumab on tumor growth and spontaneous metastasis were measured in a humanized mouse model of breast cancer bone metastasis. Effects of tumor cell–derived IL1B on bone colonization and parameters associated with metastasis were measured in MDA-MB-231, MCF7, and T47D cells transfected with IL1B/control. In tissue samples from >1,300 patients with stage II/III breast cancer, IL1B in tumor cells correlated with relapse in bone (HR = 1.85; 95% CI, 1.05–3.26; P = 0.02) and other sites (HR = 2.09; 95% CI, 1.26–3.48; P = 0.0016). In a humanized model of spontaneous breast cancer metastasis to bone, Anakinra or canakinumab reduced metastasis and reduced the number of tumor cells shed into the circulation. Production of IL1B by tumor cells promoted epithelial-to-mesenchymal transition (altered E-Cadherin, N-Cadherin, and G-Catenin), invasion, migration, and bone colonization. Contact between tumor and osteoblasts or bone marrow cells increased IL1B secretion from all three cell types. IL1B alone did not stimulate tumor cell proliferation. Instead, IL1B caused expansion of the bone metastatic niche leading to tumor proliferation. Pharmacologic inhibition of IL1B has potential as a novel treatment for breast cancer metastasis.

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