American Association for Cancer Research
10780432ccr160742-sup-164032_2_supp_3654750_tdmnls.png (479.72 kB)

Supplementary figure 2. from A Positive Feedback Loop of lncRNA-PVT1 and FOXM1 Facilitates Gastric Cancer Growth and Invasion

Download (479.72 kB)
posted on 2023-03-31, 20:01 authored by Mi-die Xu, Yiqin Wang, Weiwei Weng, Ping Wei, Peng Qi, Qiongyan Zhang, Cong Tan, Shu-juan Ni, Lei Dong, Yusi Yang, Wanrun Lin, Qinghua Xu, Dan Huang, Zhaohui Huang, Yuqing Ma, Wei Zhang, Weiqi Sheng, Xiang Du

The effect of FOXM1-PVT1 loop on EZH2, c-Myc and NOP2 in GC AGS cells were transfected with the indicated plasmids or siRNA for 48 h. The western blot results showed the protein level of FOXM1, EZH2, c-Myc and NOP2 in each group.


Science and Technology Commission of Shanghai Municipality

National Natural Science Foundation of China

Hospital Foundation of Fudan University Shanghai Cancer Center

Shanghai Hospital Development Center

Shanghai Key Developing Disciplines

Shanghai Science and Technology Development Fund

Domestic Science and Technology Cooperation



Purpose: The long, noncoding RNA (lncRNA) PVT1 is an important epigenetic regulator with a critical role in human tumors. Here, we aimed to investigate the clinical application and the potential molecular mechanisms of PVT1 in gastric cancer tumorigenesis and progression.Experimental Design: The expression level of PVT1 was determined by RT-qPCR analysis in 190 pairs of gastric cancer tissues and adjacent normal gastric mucosa tissues (ANT). The biologic functions of PVT1 were assessed by in vitro and in vivo functional experiments. RNA protein pull-down assays and LS/MS mass spectrometry analysis were performed to detect and identify the PVT1-interacting protein FOXM1. Protein–RNA immunoprecipitation assays were conducted to examine the interaction of FOXM1 and PVT1. Chromatin immunoprecipitation (ChIP) and luciferase analyses were utilized to identify the binding site of FOXM1 on the PVT1 promoter.Results: The lncRNA PVT1 was significantly upregulated in gastric cancer tissues compared with ANTs. High expression of PVT1 predicted poor prognosis in patients with gastric cancer. PVT1 enhanced gastric cancer cell proliferation and invasion in vitro and in vivo. PVT1 directly bound FOXM1 protein and increased FOXM1 posttranslationally. Moreover, PVT1 is also a FOXM1-responsive lncRNA, and FOXM1 directly binds to the PVT1 promoter to activate its transcription. Finally, PVT1 fulfilled its oncogenic functions in a FOXM1-mediated manner.Conclusions: Our study suggests that PVT1 promotes tumor progression by interacting with FOXM1. PVT1 may be a valuable prognostic predictor for gastric cancer, and the positive feedback loop of PVT1-FOXM1 could be a therapeutic target in pharmacologic strategies. Clin Cancer Res; 23(8); 2071–80. ©2016 AACR.

Usage metrics

    Clinical Cancer Research



    Ref. manager