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Supplementary Tables 1 and Figures 1 - 9 from Prognostic Significance of CD169+ Lymph Node Sinus Macrophages in Patients with Malignant Melanoma

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posted on 2023-04-03, 23:10 authored by Yoichi Saito, Koji Ohnishi, Azusa Miyashita, Satoshi Nakahara, Yukio Fujiwara, Hasita Horlad, Takanobu Motoshima, Satoshi Fukushima, Masatoshi Jinnin, Hironobu Ihn, Motohiro Takeya, Yoshihiro Komohara

Table S1. Additional univariate (A) and multivariate (B) Cox regression analyses of potential prognostic factors for overall survival. Figure S1. Decolorized sections. Immunostaining of the negative control melanomas and RLNs treated with or without melanin bleaching. Figure S2. Hematoxylin-eosin staining of the sinus areas in the RLNs. The lower images are magnifications of the squares in the upper images. Arrows indicate RLN sinus areas. Figure S3. Immunohistochemical analysis of CD169+, CD68+ and CD163+ macrophages in the RLNs. Higher magnification images of Figure 1B. Figure S4. The expression of CD14 in the sinus macrophages in the RLNs. An immunostaining of CD14 in the RLNs. Figure S5. Overall Kaplan-Meier survival curves for the 84 melanoma patients as related to the clinicopathological features. Figure S6. The expression of TIA-1 in infiltrating CD8+ T cells in the melanomas. An immunostaining of TIA-1 in the melanomas. The lower image is a magnified view of the square in the upper image. Figure S7. The expression of granzyme B in infiltrating CD8+ T cells in the melanomas. An immunostaining of Granzyme B in the melanomas. The lower image is a magnified view of the square in the upper image. Figure S8. The expression of CD43, the major ligand for CD169, in the sinus macrophages in the RLNs. An immunostaining of CD43 in the RLNs. The lower image is a magnified view of the square in the upper image. Figure S9. Flow cytometry of CD169 and costimulatory molecules on day 3 in macrophages cultured with or without IFN-alpha stimulation during the last 24 hours of culture. One representative result (of three) is shown. The black dashed lines in the histograms represent isotype controls.

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ARTICLE ABSTRACT

CD169 (sialoadhesin) is a sialic acid receptor that is specifically expressed on macrophages, including lymph node sinus macrophages. Animal studies suggest that CD169+ macrophages in lymph nodes have properties in preventing cancers. In order to determine the significance of CD169+ macrophages in patients with malignant melanoma, we evaluated tissue samples from 93 patients to investigate CD169 expression in regional lymph nodes (RLN) and determine the relationship of this expression with overall survival and various clinicopathologic factors. Higher densities of CD169+ cells were significantly associated with longer overall survival (P = 0.001). A multivariate analysis showed that the density of CD169+ cells was an independent prognostic factor, with higher densities correlating with higher density of CD8+ cytotoxic T cells within tumor sites. High CD169 expression in macrophages could be stimulated by IFNα in vitro, and in RLNs, IFNα-producing macrophages and CD303+ plasmacytoid dendritic cells were identified surrounding CD169+ macrophages. These data suggest that IFNα-stimulated CD169+ macrophages in RLNs are closely involved in T-cell–mediated antitumor immunity and may be a useful marker for assessing the clinical prognosis and monitoring antitumor immunity in patients with malignant melanoma. Cancer Immunol Res; 3(12); 1356–63. ©2015 AACR.

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