Supplementary Tables 1 and Figures 1 - 9 from Prognostic Significance of CD169⁺ Lymph Node Sinus Macrophages in Patients with Malignant Melanoma

Table S1. Additional univariate (A) and multivariate (B) Cox regression analyses of potential prognostic factors for overall survival. Figure S1. Decolorized sections. Immunostaining of the negative control melanomas and RLNs treated with or without melanin bleaching. Figure S2. Hematoxylin-eosin staining of the sinus areas in the RLNs. The lower images are magnifications of the squares in the upper images. Arrows indicate RLN sinus areas. Figure S3. Immunohistochemical analysis of CD169+, CD68+ and CD163+ macrophages in the RLNs. Higher magnification images of Figure 1B. Figure S4. The expression of CD14 in the sinus macrophages in the RLNs. An immunostaining of CD14 in the RLNs. Figure S5. Overall Kaplan-Meier survival curves for the 84 melanoma patients as related to the clinicopathological features. Figure S6. The expression of TIA-1 in infiltrating CD8+ T cells in the melanomas. An immunostaining of TIA-1 in the melanomas. The lower image is a magnified view of the square in the upper image. Figure S7. The expression of granzyme B in infiltrating CD8+ T cells in the melanomas. An immunostaining of Granzyme B in the melanomas. The lower image is a magnified view of the square in the upper image. Figure S8. The expression of CD43, the major ligand for CD169, in the sinus macrophages in the RLNs. An immunostaining of CD43 in the RLNs. The lower image is a magnified view of the square in the upper image. Figure S9. Flow cytometry of CD169 and costimulatory molecules on day 3 in macrophages cultured with or without IFN-alpha stimulation during the last 24 hours of culture. One representative result (of three) is shown. The black dashed lines in the histograms represent isotype controls.