American Association for Cancer Research
Browse
10780432ccr151416-sup-151322_1_table_3182994_nw647w.pptx (63.21 kB)

Supplementary Table 1 from Enumeration and Molecular Characterization of Tumor Cells in Lung Cancer Patients Using a Novel In Vivo Device for Capturing Circulating Tumor Cells

Download (63.21 kB)
figure
posted on 2023-03-31, 18:20 authored by Tobias M. Gorges, Nicole Penkalla, Thomas Schalk, Simon A. Joosse, Sabine Riethdorf, Johannes Tucholski, Klaus Lücke, Harriet Wikman, Stephen Jackson, Nora Brychta, Oliver von Ahsen, Christian Schumann, Thomas Krahn, Klaus Pantel

Supplementary Table 1. Additional table showing summarized patient data correlated to the appearance of CTCs.

Funding

BMBF

Ministerium für Wirtschaft des Landes Brandenburg und der

ERC

History

ARTICLE ABSTRACT

Purpose: The use of circulating tumor cells (CTC) as “liquid biopsy” is limited by the very low yield of CTCs available for subsequent analyses. Most in vitro approaches rely on small sample volumes (5–10 mL).Experimental Design: Here, we used a novel approach, the GILUPI CellCollector, which enables an in vivo isolation of CTCs from peripheral blood. In total, 50 lung cancer patients were screened in two subsequent device applications before and after therapy (n = 185 applications).Results: By in vivo isolation, 58% (108/185) of the patients were positive for ≥1 CTC (median, 5 CTCs; range, 1–56 cells) as compared with 27% (23/84; range, 1–300 cells) using the FDA-cleared CellSearch system. Furthermore, we could show that treatment response during therapy was associated with significant decreases in CTC counts (P = 0.001). By dPCR, mutations in the KRAS and EGFR genes relevant for treatment decisions could be detected in CTCs captured by in vivo isolation and confirmed in the primary tumors of the same patients.Conclusions: In vivo isolation of CTCs overcomes blood volume limitations of other approaches, which might help to implement CTC-based “liquid biopsies” into clinical decision making. Clin Cancer Res; 22(9); 2197–206. ©2015 AACR.