American Association for Cancer Research
ccr-22-3815_supplementary_figures_s1-s4_suppfs1-4.pptx (4.39 MB)

Supplementary Figures S1-S4 from The Prognostic Effect of Immune Cell Infiltration Depends on Molecular Subtype in Endometrioid Ovarian Carcinomas

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posted on 2023-09-01, 08:22 authored by Karolin Heinze, Evan S. Cairns, Shelby Thornton, Bronwyn Harris, Katy Milne, Marcel Grube, Charlotte Meyer, Anthony N. Karnezis, Sian Fereday, Dale W. Garsed, Samuel C.Y. Leung, Derek S. Chiu, Malak Moubarak, Philipp Harter, Florian Heitz, Jessica N. McAlpine, Anna DeFazio, David D.L. Bowtell, Ellen L. Goode, Malcolm Pike, Susan J. Ramus, C. Leigh Pearce, Annette Staebler, Martin Köbel, Stefan Kommoss, Aline Talhouk, Brad H. Nelson, Michael S. Anglesio

Supplementary Figures S1-S4


Deutsche Forschungsgemeinschaft (DFG)

Janet D. Cottrelle Foundation (JDCF)

Michael Smith Health Research BC (MSFHR)

BC Cancer Foundation

VGH and UBC Hospital Foundation (VGH & UBC Hospital Foundation)

National Health and Medical Research Council (NHMRC)

National Cancer Institute (NCI)

United States Department of Health and Human Services

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U.S. Army Medical Research and Development Command (MRDC)

Victorian Cancer Agency (VCA)



Endometrioid ovarian carcinoma (ENOC) is the second most-common type of ovarian carcinoma, comprising 10%–20% of cases. Recently, the study of ENOC has benefitted from comparisons to endometrial carcinomas including defining ENOC with four prognostic molecular subtypes. Each subtype suggests differential mechanisms of progression, although tumor-initiating events remain elusive. There is evidence that the ovarian microenvironment may be critical to early lesion establishment and progression. However, while immune infiltrates have been well studied in high-grade serous ovarian carcinoma, studies in ENOC are limited. We report on 210 ENOC, with clinical follow-up and molecular subtype annotation. Using multiplex IHC and immunofluorescence, we examine the prevalence of T-cell lineage, B-cell lineage, macrophages, and populations with programmed cell death protein 1 or programmed death-ligand 1 across subtypes of ENOC. Immune cell infiltrates in tumor epithelium and stroma showed higher densities in ENOC subtypes with known high mutation burden (POLEmut and MMRd). While molecular subtypes were prognostically significant, immune infiltrates were not (overall survival P > 0.2). Analysis by molecular subtype revealed that immune cell density was prognostically significant in only the no specific molecular profile (NSMP) subtype, where immune infiltrates lacking B cells (TILB minus) had inferior outcome (disease-specific survival: HR, 4.0; 95% confidence interval, 1.1–14.7; P < 0.05). Similar to endometrial carcinomas, molecular subtype stratification was generally superior to immune response in predicting outcomes. Subtype stratification is critical for better understanding of ENOC, in particular the distribution and prognostic significance of immune cell infiltrates. The role of B cells in the immune response within NSMP tumors warrants further study.

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