Supplementary Figures 1 through 4 and Supplementary Materials and Methods from Antileukemic Activity of 2-Deoxy-d-Glucose through Inhibition of N-Linked Glycosylation in Acute Myeloid Leukemia with FLT3-ITD or c-KIT Mutations
posted on 2023-04-03, 15:25authored byClément Larrue, Estelle Saland, François Vergez, Nizar Serhan, Eric Delabesse, Véronique Mansat-De Mas, Marie-Anne Hospital, Jérôme Tamburini, Stéphane Manenti, Jean Emmanuel Sarry, Christian Récher
This file contains additional figures that enrich results presented in the main manuscript. We evaluated the impact of the 2-DG on the expression of both wild-type FLT3 and KIT. Then, we excluded a potential role for cellular protein degradation pathways such as apoptosis or ubiquitin-proteasome pathway in response to 2-DG. We also investigated the subcellular location of FLT3 after 2-DG treatment. In addition, we assessed the impact of the 2-DG on the phosphorylation of Lyn and Akt, Finally, this file contains data on the effects of 2-DG in a rare primary sample patient carrying the FLT3-TKD mutation. Supplementary material and methods for qRT-PCR.