Supplementary Figures 1-13 from PEST Domain Mutations in Notch Receptors Comprise an Oncogenic Driver Segment in Triple-Negative Breast Cancer Sensitive to a γ-Secretase Inhibitor

<p>Supplementary Figures 1-13 Supplementary Figure 1: Simple mutations and copy number alterations in NOTCH1, NOTCH2 and NOTCH3. Supplementary Figure 2: Simple mutations and copy number alterations in NOTCH4. Supplementary Figure 3: Complex alterations identified by TopNotch Supplementary Figure 4: Intra-NOTCH1 deletion in TCGA-BH-A1FC. Supplementary Figure 5: BRD4-NOTCH3 fusion and PEST domain deletion in TCGA-AN-A0AR. Supplementary Figure 6: Three PEST domain alterations identified in the TCGA breast cancer dataset. Supplementary Figure 7: Notch altered tumors often exhibit increased pathway activity. Supplementary Figure 8: Notch aberrations in pre-clinical GSI sensitive models. Supplementary Figure 9: NOTCH1 aberrations in the HBCx-14 breast cancer PDX model. Supplementary Figure 10: Tumor growth inhibition plots of PF-03084014 in Notch wt models Supplementary Figure 11: Comparison of NICD1 between Notch wt and mutant models. Supplementary Figure 12: Quantitative RT-PCR of HES and HEY target genes in Notch wt and mutant xenograft models Supplementary Figure 13:Quantitative RT-PCR of Notch target genes in Notch wt and mutant xenograft models</p>