Supplementary Figure S9 from LNS8801 inhibits Acute Myeloid Leukemia by Inducing the Production of Reactive Oxygen Species and Activating the Endoplasmic Reticulum Stress Pathway
Supplementary Figure S9. Mcl-1 is not responsible for LNS8801-induced death in AML (A) Proliferation assay dose curve of AML cells treated with the Mcl-1 specific inhibitor S63845. Cells were treated with drug for 72 hours. 6 replicates were used per dose. (B) Western blot of U937 cells overexpressing Mcl-1, treated with 250nM LNS8801 for 24 hours. (C) Annexin V flow analysis of U937 cells overexpressing Mcl-1. Cells were treated for 72 hours. 3 replicates were used per condition. Two-way ANOVA was used for statistical analysis for Annexin V+ flow cytometry comparing wild-type and Mcl-1 overexpressing U937 cells.