American Association for Cancer Research
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Supplementary Figure S6 from TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation

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posted on 2023-03-30, 23:20 authored by David J. Drasin, Anna L. Guarnieri, Deepika Neelakantan, Jihye Kim, Joshua H. Cabrera, Chu-An Wang, Vadym Zaberezhnyy, Pierluigi Gasparini, Luciano Cascione, Kay Huebner, Aik-Choon Tan, Heide L. Ford

Supplementary Figure S6 MiR-424 is post-transcriptionally regulated in vitro. Primary and mature miR-424 levels, by qPCR, in (A) HMLE cells constitutively expressing SNAI1 and in (B) HMLER cells constitutively expressing TWIST1 or SNAI1, after passaging the cell lines for longer than 20 days. SEM shown, n{greater than or equal to}3. *p<0.05, **p<0.01. (A) Two-tailed unpaired t-test and (B) one-way ANOVA with Bonferroni post-test.



Epithelial-to-mesenchymal transition (EMT) is a dynamic process that relies on cellular plasticity. Recently, the process of an oncogenic EMT, followed by a reverse mesenchymal-to-epithelial transition (MET), has been implicated as critical in the metastatic colonization of carcinomas. Unlike governance of epithelial programming, regulation of mesenchymal programming is not well understood in EMT. Here, we describe and characterize the first microRNA that enhances exclusively mesenchymal programming. We demonstrate that miR-424 is upregulated early during a TWIST1 or SNAI1-induced EMT, and that it causes cells to express mesenchymal genes without affecting epithelial genes, resulting in a mixed/intermediate EMT. Furthermore, miR-424 increases motility, decreases adhesion, and induces a growth arrest, changes associated with a complete EMT that can be reversed when miR-424 expression is lowered, concomitant with an MET-like process. Breast cancer patient miR-424 levels positively associate with TWIST1/2 and EMT-like gene signatures, and miR-424 is increased in primary tumors versus matched normal breast. However, miR-424 is downregulated in patient metastases versus matched primary tumors. Correspondingly, miR-424 decreases tumor initiation and is posttranscriptionally downregulated in macrometastases in mice, suggesting the need for biphasic expression of miR-424 to transit the EMT–MET axis. Next-generation RNA sequencing revealed miR-424 regulates numerous EMT and cancer stemness-associated genes, including TGFBR3, whose downregulation promotes mesenchymal phenotypes, but not tumor-initiating phenotypes. Instead, we demonstrate that increased MAPK–ERK signaling is critical for miR-424–mediated decreases in tumor-initiating phenotypes. These findings suggest miR-424 plays distinct roles in tumor progression, potentially facilitating earlier, but repressing later, stages of metastasis by regulating an EMT–MET axis. Cancer Res; 75(9); 1908–21. ©2015 AACR.

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