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Supplementary Figure S4 from Cellular Prion Protein PrPC and Ecto-5′-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy

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posted on 2023-03-31, 00:31 authored by Patrícia H. Domingues, Lalitha S.Y. Nanduri, Katarzyna Seget, Sharavan V. Venkateswaran, David Agorku, Cristina Viganó, Conrad von Schubert, Erich A. Nigg, Charles Swanton, Rocío Sotillo, Andreas Bosio, Zuzana Storchová, Olaf Hardt

Effect of ER stress on CD73, CD230, CD47, CD49b, CD55 and CD95 expression in parental and aneuploid cell lines. Ratios of cells treated with multiple ER stress inducers - glucose-deprivation (black bars), brefeldin A (BFA; red bars) 1ug/mL, thapsigargin (Thaps; blue bars) 0.25ug/ml or tunicamycin (TuniM; green bars) 2.5ug/mL for 48h - and control cells are shown for the mean fluorescence intensity values (top graph) and percentage of positive cells (bottom graph).

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European Union Seventh Framework Programme

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ARTICLE ABSTRACT

Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of approximately 300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples, and mouse models. Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrPC and the immunosuppressive cell surface enzyme ecto-5′-nucleotidase CD73. Functional analyses associated these alterations with increased cellular stress. An increased number of CD73+ cells was observed in confluent cultures in aneuploid cells relative to their diploid counterparts. An elevated expression in CD230/PrPC was observed in serum-deprived cells in association with increased generation of reactive oxygen species. Overall, our work identified biomarkers of aneuploid karyotypes, which suggest insights into the underlying molecular physiology of aneuploid cells. Cancer Res; 77(11); 2914–26. ©2017 AACR.

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