23266066cir190107-sup-217206_2_supp_5812586_pyrm0g.png (81.1 kB)

Supplementary Figure S3 from Direct Detection and Quantification of Neoantigens

figure

posted on 2023-04-04, 00:46 authored by Qing Wang, Jacqueline Douglass, Michael S. Hwang, Emily Han-Chung Hsiue, Brian J. Mog, Ming Zhang, Nickolas Papadopoulos, Kenneth W. Kinzler, Shibin Zhou, Bert Vogelstein

Sanger sequencing to confirm G12D mutation from KRAS G12D-knockin cell line.

Funding

Cancer Research

The Research Council of Norway

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Pancreatic Cancer Research

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ARTICLE ABSTRACT

Many immunotherapeutic approaches under development rely on T-cell recognition of cancer-derived peptides bound to human leukocyte antigen molecules on the cell surface. Direct experimental demonstration that such peptides are processed and bound is currently challenging. Here, we describe a method that meets this challenge. The method entailed an optimized immunoprecipitation protocol coupled with two-dimensional chromatography and mass spectrometry. The ability to detect and quantify minute amounts of predefined antigens should be useful both for basic research in tumor immunology and for the development of rationally designed cancer vaccines.