Supplementary Figure S2 from EGFR Inhibition by Cetuximab Modulates Hypoxia and IFN Response Genes in Head and Neck Squamous Cell Carcinoma

(A, B) Representative mIHC images showing staining of nuclei (blue), tumor cell (orange - PCK) and selected immune checkpoint markers in each molecular subgroup. (A) Images show four lymphoid markers - CD3+ T-cells (Green), CD8+ cytotoxic T-cells (Yellow), CD69+ activated T-cells (Red) and CD103+ resident memory T-cells (Cyan). (B) Images show three myeloid markers – HLA-DR+ APCs (Green), MHCII+ APCs (Cyan) and CD163+ M2 macrophages (Yellow). (C) Linear model showing correlation between PD-L1 CPS and Immune, Mixture and Hypoxia subgroups. (D-E) Boxplots showing cell counts distribution of different immune markers among the primary (n = 63) and recurrent (n = 20) tumors in TMA mIHC cohort. Students t-tests were used to test for significant differences between the groups.