Supplementary Figure 3 from LASP1 Is a HIF1α Target Gene Critical for Metastasis of Pancreatic Cancer

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posted on 2023-03-30, 23:00 authored by Tiansuo Zhao, He Ren, Jing Li, Jing Chen, Huan Zhang, Wen Xin, Yan Sun, Lei Sun, Yongwei Yang, Junwei Sun, Xiuchao Wang, Song Gao, Chongbiao Huang, Huafeng Zhang, Shengyu Yang, Jihui Hao

(A) The morphology of Panc-1/pLV vector and Panc-1/pLV LASP-1 cells. (B) LASP-1 expression determined by Western blot analysis in Panc-1/pLV vector andPanc-1/pLV LASP-1 cells.

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ARTICLE ABSTRACT

LASP1 is an actin-binding protein associated with actin assembly dynamics in cancer cells. Here, we report that LASP1 is overexpressed in pancreatic ductal adenocarcinoma (PDAC) where it promotes invasion and metastasis. We found that LASP1 overexpression in PDAC cells was mediated by HIF1α through direct binding to a hypoxia response element in the LASP1 promoter. HIF1α stimulated LASP1 expression in PDAC cells in vitro and mouse tumor xenografts in vivo. Clinically, LASP1 overexpression in PDAC patient specimens was associated significantly with lymph node metastasis and overall survival. Overall, our results defined LASP1 as a direct target gene for HIF1α upregulation that is critical for metastatic progression of PDAC. Cancer Res; 75(1); 111–9. ©2014 AACR.