Supplementary Figure 2 from Tumor-Induced Pressure in the Bone Microenvironment Causes Osteocytes to Promote the Growth of Prostate Cancer Bone Metastases

Supplementary Figure 2: MLO-Y4 CM promotes PCa viability, migration, and invasion. CM was isolated from MLO-Y4 cells. Viability of PCa cells after 24 hour incubation with MLO-Y4 CM as measured by resazurin. Student's two-tailed unpaired t-test was used to assess statistical differences; p<0.05. CM was also used as a chemoattractant in Boyden chamber assays to assess PCa migration and invasion. CM was compared to negative control (RPMI with 0.1% FBS) and positive control (RPMI with 10% FBS) media. One-way ANOVA with Bonferroni post-test used for analysis; bars represent significant differences where p<0.05.