posted on 2023-03-30, 23:43authored byBarbara Herkert, Audrey Kauffmann, Sandra Mollé, Christian Schnell, Thomas Ferrat, Hans Voshol, Janina Juengert, Hélène Erasimus, Grégory Marszalek, Malika Kazic-Legueux, Eric Billy, David Ruddy, Mark Stump, Daniel Guthy, Mitko Ristov, Keith Calkins, Sauveur-Michel Maira, William R. Sellers, Francesco Hofmann, Michael N. Hall, Saskia M. Brachmann
PI3Kβ inhibition induces p85/IRS2 interaction in a time-dependent manner in the PTENLOF/BRAFMUT melanoma cell line RVH-421 that is mediated by negative feedback signaling. A) Co-immunoprecipitation experiments were carried out using cell lysates of RVH-421 treated with the PI3Kbi for the indicated time and blotted for p85, and pTyr (IRS2). B)-D) Input lysates of the immunoprecipitation experiments shown in Figure 4 were immunoblotted with the indicated phospho-specific or total target protein antibodies. PI3Kβi=rac-KIN-193, mTORi=AZD8055, mTORCi=RAD001, PDK1i=GSK-2334470, AKTi=MK2206