American Association for Cancer Research
00085472can151538-sup-150746_2_supp_3316969_v17n0x.pptx (3.91 MB)

Supplemental Figures S1-S9 from Immunoregulatory Protein B7-H3 Reprograms Glucose Metabolism in Cancer Cells by ROS-Mediated Stabilization of HIF1α

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posted on 2023-03-31, 00:05 authored by Sangbin Lim, Hao Liu, Luciana Madeira da Silva, Ritu Arora, Zixing Liu, Joshua B. Phillips, David C. Schmitt, Tung Vu, Steven McClellan, Yifeng Lin, Wensheng Lin, Gary A. Piazza, Oystein Fodstad, Ming Tan

Fig. S1: Glucose uptake and lactate production in B7-H3 knockdown Fig. S2: Growth inhibition W/O B7-H3 Fig S3: Proliferation assay in glucose deprivation. Fig. S4: Expression in B7-H3 knockdown w/o CoCl2. Fig. S5: mRNA levels. Fig. S6: Reduction of ROS by ROS scavenger in B7-H3 overexpression. Fig. S7: G6PD and SOD activity. Fig. S8: Growth Inhibition by ROS scavenger. Fig. S9: Immunohistochemistry analysis of xenograft tumors.


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B7-H3 is a member of B7 family of immunoregulatory transmembrane glycoproteins expressed by T cells. While B7-H3 overexpression is associated with poor outcomes in multiple cancers, it also has immune-independent roles outside T cells and its precise mechanistic contributions to cancer are unclear. In this study, we investigated the role of B7-H3 in metabolic reprogramming of cancer cells in vitro and in vivo. We found that B7-H3 promoted the Warburg effect, evidenced by increased glucose uptake and lactate production in B7-H3–expressing cells. B7-H3 also increased the protein levels of HIF1α and its downstream targets, LDHA and PDK1, key enzymes in the glycolytic pathway. Furthermore, B7-H3 promoted reactive oxygen species–dependent stabilization of HIF1α by suppressing the activity of the stress-activated transcription factor Nrf2 and its target genes, including the antioxidants SOD1, SOD2, and PRX3. Metabolic imaging of human breast cancer xenografts in mice confirmed that B7-H3 enhanced tumor glucose uptake and tumor growth. Together, our results illuminate the critical immune-independent contributions of B7-H3 to cancer metabolism, presenting a radically new perspective on B7 family immunoregulatory proteins in malignant progression. Cancer Res; 76(8); 2231–42. ©2016 AACR.

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