10780432ccr140209-sup-126190_1_supp_2540467_n7y2k5.png (30.85 kB)
Supplemental Figure S1 from The Renin–Angiotensin System Mediates EGF Receptor–Vitamin D Receptor Cross-Talk in Colitis-Associated Colon Cancer
figure
posted on 2023-03-31, 18:24 authored by Urszula Dougherty, Reba Mustafi, Farhana Sadiq, Anas Almoghrabi, Devkumar Mustafi, Maggi Kreisheh, Sumana Sundaramurthy, Weicheng Liu, Vani J. Konda, Joel Pekow, Sharad Khare, John Hart, Loren Joseph, Alice Wyrwicz, Gregory S. Karczmar, Yan Chun Li, Marc BissonnetteSupplemental Figure S1. Tarceva, an EGFR inhibitor, blocks Ang II induced HT29 cell proliferation. Cells (5,000) were plated on 96 well plates and allowed to adhere overnight. Cells were then treated with Tarceva (10 µM), or vehicle for 2 hrs followed by Ang II (50 nM) or vehicle for 24 hrs. Cell proliferation was determined by Wst1 assay as described in the "Materials and Methods" (*,†p<0.05, compared to control; n=2 independent platings).
History
ARTICLE ABSTRACT
Purpose: We previously showed that EGF receptor (EGFR) promotes tumorigenesis in the azoxymethane/dextran sulfate sodium (AOM/DSS) model, whereas vitamin D suppresses tumorigenesis. EGFR–vitamin D receptor (VDR) interactions, however, are incompletely understood. Vitamin D inhibits the renin–angiotensin system (RAS), whereas RAS can activate EGFR. We aimed to elucidate EGFR–VDR cross-talk in colorectal carcinogenesis.Experimental Design: To examine VDR–RAS interactions, we treated Vdr+/+ and Vdr−/− mice with AOM/DSS. Effects of VDR on RAS and EGFR were examined by Western blotting, immunostaining, and real-time PCR. We also examined the effect of vitamin D3 on colonic RAS in Vdr+/+ mice. EGFR regulation of VDR was examined in hypomorphic EgfrWaved2 (Wa2) and Egfrwild-type mice. Angiotensin II (Ang II)–induced EGFR activation was studied in cell culture.Results:Vdr deletion significantly increased tumorigenesis, activated EGFR and β-catenin signaling, and increased colonic RAS components, including renin and angiotensin II. Dietary VD3 supplementation suppressed colonic renin. Renin was increased in human colon cancers. In studies in vitro, Ang II activated EGFR and stimulated colon cancer cell proliferation by an EGFR-mediated mechanism. Ang II also activated macrophages and colonic fibroblasts. Compared with tumors from EgfrWaved2 mice, tumors from Egfrwild-type mice showed upregulated Snail1, a suppressor of VDR, and downregulated VDR.Conclusions: VDR suppresses the colonic RAS cascade, limits EGFR signals, and inhibits colitis-associated tumorigenesis, whereas EGFR increases Snail1 and downregulates VDR in colonic tumors. Taken together, these results uncover a RAS-dependent mechanism mediating EGFR and VDR cross-talk in colon cancer. Clin Cancer Res; 20(22); 5848–59. ©2014 AACR.Usage metrics
Categories
Keywords
Cancer PreventionAnimal models of preventionCarcinogenesisAnimal models of carcinogenesisCell CycleSignal transduction pathwaysCell SignalingAutocrine-paracrine signalingEndocrinologyGrowth factors and receptorsGastrointestinal CancersColorectal cancerPreclinical ModelsAnimal models of cancerProgression, Invasion & MetastasisInflammation and tumor developmentTumor MicroenvironmentTumor-stromal cell interactions
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC