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posted on 2023-04-03, 15:46 authored by Alicia M. Waters, Jerry E. Stewart, Venkatram R. Atigadda, Elizabeth Mroczek-Musulman, Donald D. Muccio, Clinton J. Grubbs, Elizabeth A. Beierle <p>Cellular proliferation was measured with trypan blue exclusion. Cell lines were treated with UAB30 at 0 or 10 μM for 24 hrs. Cells were stained with trypan blue and counted with a hemacytometer. A The total number of cells decreased in all 3 cell lines, but this decrease did not reach statistical significance. B However, when the ratio between the dead : viable cells was examined, this ratio was significantly increased indicating that the cells were not proliferating.</p>
Funding
Cohen Research Acceleration and Innovation Fund
National Cancer Institute
UAB
History
ARTICLE ABSTRACT
Rare tumors of solid organs remain some of the most difficult pediatric cancers to cure. These difficult tumors include rare pediatric renal malignancies, such as malignant rhabdoid kidney tumors (MRKT) and non-osseous renal Ewing sarcoma, and hepatoblastoma, a pediatric liver tumor that arises from immature liver cells. There are data in adult renal and hepatic malignancies demonstrating the efficacy of retinoid therapy. The investigation of retinoic acid therapy in cancer is not a new strategy, but the widespread adoption of this therapy has been hindered by toxicities. Our laboratory has been investigating a novel synthetic rexinoid, UAB30, which exhibits a more favorable side-effect profile. In this study, we hypothesized that UAB30 would diminish the growth of tumor cells from both rare renal and liver tumors in vitro and in vivo. We successfully demonstrated decreased cellular proliferation, invasion and migration, cell-cycle arrest, and increased apoptosis after treatment with UAB30. Additionally, in in vivo murine models of human hepatoblastoma or rare human renal tumors, there were significantly decreased tumor xenograft growth and increased animal survival after UAB30 treatment. UAB30 should be further investigated as a developing therapeutic in these rare and difficult-to-treat pediatric solid organ tumors. Mol Cancer Ther; 15(5); 911–21. ©2016 AACR.
