American Association for Cancer Research
10780432ccr151899-sup-153647_1_supp_3190330_nwh26f.pptx (1.86 MB)

Supplemental Figure 2 from The Oncolytic Adenovirus VCN-01 as Therapeutic Approach Against Pediatric Osteosarcoma

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posted on 2023-03-31, 18:22 authored by Naiara Martínez-Vélez, Enric Xipell, Beatriz Vera, Arlet Acanda de la Rocha, Marta Zalacain, Lucía Marrodán, Marisol Gonzalez-Huarriz, Gemma Toledo, Manel Cascallo, Ramón Alemany, Ana Patiño, Marta M. Alonso

Supplemental Figure 2


European Union

Instituto de Salud Carlos III

Spanish Ministry of Science and Innovation


The Department of Health of the Government of Navarra

The Basque Foundation for Health Research

Fundacíon Caja Navarra

Credit Andorra Foundation


United States Department of the Air Force

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Spanish Ministry of Education and Science



Purpose: Osteosarcoma is the most common malignant bone tumor in children and adolescents. Despite aggressive chemotherapy, more than 30% of patients do not respond and develop bone or lung metastasis. Oncolytic adenoviruses engineered to specifically destroy cancer cells are a feasible option for osteosarcoma treatment. VCN-01 is a replication-competent adenovirus specifically engineered to replicate in tumors with a defective RB pathway, presents an enhanced infectivity through a modified fiber and an improved distribution through the expression of a soluble hyaluronidase. The aim of this study is to elucidate whether the use of VCN-01 would be an effective therapeutic strategy for pediatric osteosarcoma.Experimental Design: We used osteosarcoma cell lines established from patients with metastatic disease (531MII, 678R, 588M, and 595M) and a commercial cell line (143B). MTT assays were carried out to evaluate the cytotoxicity of VCN-01. Hexon assays were used to evaluate the replication of the virus. Western blot analysis was performed to assess the expression levels of viral proteins and autophagic markers. The antitumor effect of VCN-01 was evaluated in orthotopic and metastatic osteosarcoma murine animal models.Results: This study found that VCN-01, a new generation genetically modified oncolytic adenovirus, administered locally or systemically, had a potent antisarcoma effect in vitro and in vivo in mouse models of intratibial and lung metastatic osteosarcoma. Moreover, VCN-01 administration showed a safe toxicity profile.Conclusions: These results uncover VCN-01 as a promising strategy for osteosarcoma, setting the bases to propel a phase I/II trial for kids with this disease. Clin Cancer Res; 22(9); 2217–25. ©2015 AACR.