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Supplemental Figure 1 from Lifetime Exposure to Cigarette Smoke, B-Cell Tumor Immune Infiltration, and Immunoglobulin Abundance in Ovarian Tumors

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posted on 2024-06-03, 07:42 authored by Cassandra A. Hathaway, Mary K. Townsend, Tianyi Wang, Christine Vinci, Danielle E. Jake-Schoffman, Jonathan L. Hecht, Daryoush Saeed-Vafa, Carlos Moran Segura, Jonathan V. Nguyen, Jose R. Conejo-Garcia, Brooke L. Fridley, Shelley S. Tworoger

Supplemental Figure 1: Flow chart for the selection of ovarian cancer cases in the Nurses’ Health Study (NHS) and NHSII, limited to ovarian cancer diagnosis, available tissue, available data on parental smoke exposure, and successful multiplex immunofluorescence staining.

Funding

National Cancer Institute (NCI)

United States Department of Health and Human Services

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Florida Department of Health (DOH)

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ARTICLE ABSTRACT

Cigarette smoke exposure has been linked to systemic immune dysfunction, including for B-cell and immunoglobulin (Ig) production, and poor outcomes in patients with ovarian cancer. No study has evaluated the impact of smoke exposure across the life-course on B-cell infiltration and Ig abundance in ovarian tumors. We measured markers of B and plasma cells and Ig isotypes using multiplex immunofluorescence on 395 ovarian cancer tumors in the Nurses’ Health Study (NHS)/NHSII. We conducted beta-binomial analyses evaluating odds ratios (OR) and 95% confidence intervals (CI) for positivity of immune markers by cigarette exposure among cases and Cox proportional hazards models to evaluate hazard ratios (HR) and 95% CI for developing tumors with low (