posted on 2023-03-31, 20:09authored byQinghai Zeng, Jianye Liu, Peiguo Cao, Jingjing Li, Xiaoming Liu, Xiaojun Fan, Ling Liu, Yan Cheng, Wei Xiong, Jigang Li, Hao Bo, Yuxing Zhu, Fei Yang, Jun Hu, Ming Zhou, Yanhong Zhou, Qiong Zou, Jianda Zhou, Ke Cao
Restoration of REDD1 expression counteracts miR-22-mediated Taxol sensitivity. EJ and BIU87 cells were treated with Taxol and combinations of miR-22 mimics, miR-22 antagonist, shRNA-REDD1, and REDD1 overexpression vector. (A) Protein expression of REDD1, EEF2K, c-caspase 3, c-PARP, and LC3-I/II was determined by western blot. (B) CCK-8 was used to determine treatment effect on cell proliferation. (C) Flow cytometry was used to detect cell apoptosis. (D) Autophagy was assessed by immunofluorescence of LCB3 and quantification by flow cytometry. ***P<0.001, **P<0.01, *P<0.05.
Funding
National Natural Science Foundation of China
Natural Science Foundation of Hunan Province
Central South University
Huxiang Young Talent Project
Third Xiangya Hospital of Central South University